MAGED1 recombinant protein

MAGED1, 504-760aa, Human, His tag, E Coli

Cat. Num. AAA205464

• Gene Names: MAGED1; NRAGE; DLXIN-1
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: MAGED1; 504-760aa; Human; His tag; E Coli; Melanoma-associated antigen D1 isoform a; DLXIN-1; NRAGE; MAGED1 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 1mM DTT
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSLRPSPNS RASQNPGAAQ PRDVALLQER ANKLVKYLML KDYTKVPIKR SEMLRDIIRE YTDVYPEIIE RACFVLEKKF GIQLKEIDKE EHLYILISTP ESLAGILGTT KDTPKLGLLL VILGVIFMNG NRASEAVLWE ALRKMGLRPG VRHPLLGDLR KLLTYEFVKQ KYLDYRRVPN SNPPEYEFLW GLRSYHETSK MKVLRFIAEV QKRDPRDWTA QFMEAADEAL DALDAAAAEA EARAEARTRM GIGDEAVSGP
• Sequence Length: 834

• Applicable Applications for MAGED1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for MAGED1 recombinant protein

MAGED1, as known as Melanoma-associated antigen D1, is expressed in almost all normal adult tissues. It has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. It has been involved in Prader-Willi syndrome, which includes hyperphagia, repetitive and compulsive behaviors, and cognitive impairment. Recombinant human MAGED1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for MAGED1 recombinant protein

Apoptosis

References

Dombret C. et al. (2012) Hum Mol Genet. 21:4703-4717; Mouri,. et al. (2012) J Neurosci. 32:4562-4580.

NCBI and Uniprot Product Information

• NCBI GI #: 52632381
• NCBI GeneID: 9500
• NCBI Accession #: NP_001005333
• NCBI GenBank Nucleotide #: NM_001005333.1
• UniProt Accession #: Q9Y5V3; Q5VSH6; Q8IZ84; Q8WY92; Q9H352; Q9HBT4; Q9UF36
• Molecular Weight: 31.7kDa (280aa), confirmed by MALDI-TOF
• NCBI Official Full Name: melanoma-associated antigen D1 isoform a
• NCBI Official Synonym Full Names: MAGE family member D1
• NCBI Official Symbol: MAGED1
• NCBI Official Synonym Symbols: NRAGE; DLXIN-1
• NCBI Protein Information: melanoma-associated antigen D1
• UniProt Protein Name: Melanoma-associated antigen D1
• Protein Family: Melanoma-associated antigen
• UniProt Gene Name: MAGED1
• UniProt Synonym Gene Names: NRAGE

NCBI Description

This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Inhibits cell cycle progression, and facilitates NGFR-mediated apoptosis. May act as a regulator of the function of DLX family members. May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Plays a role in the circadian rhythm regulation. May act as RORA co-regulator, modulating the expression of core clock genes such as ARNTL/BMAL1 and NFIL3, induced, or NR1D1, repressed.