CA14 active protein

CA14, 16-290aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA205222

• Gene Names: CA14; CAXiV
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: CA14; 16-290aa; Human; His tag; E Coli (Bioactivity Validated); Carbonic anhydrase 14; CAXiV; CA14 active protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSADGGQHW TYEGPHGQDH WPASYPECGN NAQSPIDIQT DSVTFDPDLP ALQPHGYDQP GTEPLDLHNN GHTVQLSLPS TLYLGGLPRK YVAAQLHLHW GQKGSPGGSE HQINSEATFA ELHIVHYDSD SYDSLSEAAE RPQGLAVLGI LIEVGETKNI AYEHILSHLH EVRHKDQKTS VPPFNLRELL PKQLGQYFRY NGSLTTPPCY QSVLWTVFYR RSQISMEQLE KLQGTLFSTE EEPSKLLVQN YRALQPLNQR MVFASFIQAG SSYTTGEM
• Sequence Length: 337

• Applicable Applications for CA14 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is > 700 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CA14 active protein

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA14 is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. Recombinant human CA14 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for CA14 active protein

Neuroscience

References

Temperini C., et al. (2006) Chemistry. 12:7057-7066; Temperini C., et al. (2006) J. Med. Chem. 49:3019-3027;

NCBI and Uniprot Product Information

• NCBI GI #: 6912284
• NCBI GeneID: 23632
• NCBI Accession #: NP_036245
• NCBI GenBank Nucleotide #: NM_012113.2
• UniProt Accession #: Q9ULX7; Q5TB24; Q8NCF4
• Molecular Weight: 33.2kDa (298aa) confirmed by MALDI-TOF
• NCBI Official Full Name: carbonic anhydrase 14
• NCBI Official Synonym Full Names: carbonic anhydrase 14
• NCBI Official Symbol: CA14
• NCBI Official Synonym Symbols: CAXiV
• NCBI Protein Information: carbonic anhydrase 14
• UniProt Protein Name: Carbonic anhydrase 14
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA14
• UniProt Synonym Gene Names: CA-XIV

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA XIV is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. [provided by RefSeq, Jul 2008]

Uniprot Description

Reversible hydration of carbon dioxide.