TCEA2 recombinant protein

TCEA2,1-299aa, Human, His tag, E Coli

Cat. Num. AAA204863

• Gene Names: TCEA2; TFIIS
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: TCEA2; 1-299aa; Human; His tag; E Coli; Transcription elongation factor A protein 2 isoform a; transcription elongation factor A (SII); 2; TFIIS; TCEA2 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.25M NaCl
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMMGKEEE IARIARRLDK MVTKKSAEGA MDLLRELKAM PITLHLLQST RVGMSVNALR KQSSDEEVIA LAKSLIKSWK KLLDASDAKA RERGRGMPLP TSSRDASEAP DPSRKRPELP RAPSTPRITT FPPVPVTCDA VRNKCREMLT AALQTDHDHV AIGADCERLS AQIEECIFRD VGNTDMKYKN RVRSRISNLK DAKNPDLRRN VLCGAITPQQ IAVMTSEEMA SDELKEIRKA MTKEAIREHQ MARTGGTQTD LFTCGKCRKK NCTYTQVQTR SSDEPMTTFV VCNECGNRWK FC
• Sequence Length: 299

• Applicable Applications for TCEA2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TCEA2 recombinant protein

TCEA2 is found in the nucleus, where it functions as an SII class transcription elongation factor. Elongation factors in this class are responsible for releasing RNA polymerase II ternary complexes from transcriptional arrest at template-encoded arresting sites. The protein has been shown to interact with general transcription factor IIB, a basal transcription factor. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human TCEA2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for TCEA2 recombinant protein

Epigenomics

References

Weaver ZA, Kane CM. Mar 1998. Genomics 46 (3): 516-9.

NCBI and Uniprot Product Information

• NCBI GI #: 4507385
• NCBI GeneID: 6919
• NCBI Accession #: NP_003186
• NCBI GenBank Nucleotide #: NM_003195.5
• UniProt Accession #: Q15560; Q8TD37; Q8TD38; B3KNM1
• Molecular Weight: 36kDa (322aa), confirmed by MALDI-TOF
• NCBI Official Full Name: transcription elongation factor A protein 2 isoform a
• NCBI Official Synonym Full Names: transcription elongation factor A2
• NCBI Official Symbol: TCEA2
• NCBI Official Synonym Symbols: TFIIS
• NCBI Protein Information: transcription elongation factor A protein 2
• UniProt Protein Name: Transcription elongation factor A protein 2
• Protein Family: Tuliposide A-converting enzyme
• UniProt Gene Name: TCEA2

NCBI Description

The protein encoded by this gene is found in the nucleus, where it functions as an SII class transcription elongation factor. Elongation factors in this class are responsible for releasing RNA polymerase II ternary complexes from transcriptional arrest at template-encoded arresting sites. The encoded protein has been shown to interact with general transcription factor IIB, a basal transcription factor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.