SDS-Page
uBE2F recombinant protein
uBE2F, 1-185aa, Human, His tag, E Coli
Gene Names
UBE2F; NCE2
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
uBE2F; 1-185aa; Human; His tag; E Coli; NEDD8-conjugating enzyme uBE2F; NCE2; uBE2F recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer, pH8.0, 30% glycerol, 1mM DTT, 200mM NaCl
Concentration
1mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMLTLAS KLKRDDGLKG SRTAATASDS TRRVSVRDKL LVKEVAELEA NLPCTCKVHF PDPNKLHCFQ LTVTPDEGYY QGGKFQFETE VPDAYNMVPP KVKCLTKIWH PNITETGEIC LSLLREHSID GTGWAPTRTL KDVVWGLNSL FTDLLNFDDP LNIEAAEHHL RDKEDFRNKV DDYIKRYAR
Sequence Length
185
Applicable Applications for uBE2F recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for uBE2F recombinant protein
uBE2F, also Known as ubiquitin-conjugating enzyme E2 F, belongs to the ubiquitin-conjugating enzyme family and uBE2F subfamily. ubiquitin-conjugating enzymes more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. uBE2F accepts the ubiquitin-like protein NEDD8 from the uBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. Recombinant human uBE2F protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for uBE2F recombinant protein
References
Huang D.T., et al. (2009) Mol. Cell 33:483-495; Nandi, D. et al. (2006) Journal of biosciences 31 (1): 137-55.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23.7kDa (209aa) confirmed by MALDI-TOF
NCBI Official Full Name
NEDD8-conjugating enzyme UBE2F isoform 1
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 F (putative)
NCBI Official Symbol
UBE2F
NCBI Official Synonym Symbols
NCE2
NCBI Protein Information
NEDD8-conjugating enzyme UBE2F
UniProt Protein Name
NEDD8-conjugating enzyme UBE2F
Protein Family
UniProt Gene Name
UBE2F
UniProt Synonym Gene Names
NCE2
Uniprot Description
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Research Articles on uBE2F
Similar Products
Product Notes
The uBE2F ube2f (Catalog #AAA204718) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's uBE2F can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the uBE2F ube2f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMLTLAS KLKRDDGLKG SRTAATASDS TRRVSVRDKL LVKEVAELEA NLPCTCKVHF PDPNKLHCFQ LTVTPDEGYY QGGKFQFETE VPDAYNMVPP KVKCLTKIWH PNITETGEIC LSLLREHSID GTGWAPTRTL KDVVWGLNSL FTDLLNFDDP LNIEAAEHHL RDKEDFRNKV DDYIKRYAR. It is sometimes possible for the material contained within the vial of "uBE2F, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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