ARL4D recombinant protein

ARL4D, 1-201aa, Human, His tag, E Coli

Cat. Num. AAA204668

• Gene Names: ARL4D; ARL6; ARF4L
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: ARL4D; 1-201aa; Human; His tag; E Coli; ADP-ribosylation factor-like protein 4D; ARF4L; ARL6; ARL4D recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200mM NaCl
• Concentration: 0.25mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGNHLTEMAP TASSFLPHFQ ALHVVVIGLD SAGKTSLLYR LKFKEFVQSV PTKGFNTEKI RVPLGGSRGI TFQVWDVGGQ EKLRPLWRSY TRRTDGLVFV VDAAEAERLE EAKVELHRIS RASDNQGVPV LVLANKQDQP GALSAAEVEK RLAVRELAAA TLTHVQGCSA VDGLGLQQGL ERLYEMILKR KKAARGGKKR R
• Sequence Length: 201

• Applicable Applications for ARL4D recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ARL4D recombinant protein

ARL4D (ADP-ribosylation factor-like protein 4D) is a developmentally regulated member of the ADP-ribosylation factor/ARF-like protein (ARF/ARL) family of Ras-related GTPases. Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). This protein may play a role in membrane-associated intracellular trafficking. Mutations in this gene have been associated with Bardet-Biedl syndrome (BBS). Recombinant human ARL4D protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for ARL4D recombinant protein

Signal Transduction

References

Li CC, et al. (2007) Mol Biol Cell. 18 (11):4420-37.; Hofmann I., et al (2007) Curr. Biol. 17:711-716

NCBI and Uniprot Product Information

• NCBI GI #: 41327767
• NCBI GeneID: 379
• NCBI Accession #: NP_001652
• NCBI GenBank Nucleotide #: NM_001661.3
• UniProt Accession #: P49703; B2RC59; D3DX43
• Molecular Weight: 24.3kDa (221aa), confirmed by MALDI-TOF
• NCBI Official Full Name: ADP-ribosylation factor-like protein 4D
• NCBI Official Synonym Full Names: ADP ribosylation factor like GTPase 4D
• NCBI Official Symbol: ARL4D
• NCBI Official Synonym Symbols: ARL6; ARF4L
• NCBI Protein Information: ADP-ribosylation factor-like protein 4D
• UniProt Protein Name: ADP-ribosylation factor-like protein 4D
• Protein Family: ADP-ribosylation factor-like protein
• UniProt Gene Name: ARL4D
• UniProt Synonym Gene Names: ARF4L

NCBI Description

ADP-ribosylation factor 4D is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL4D is closely similar to ARL4A and ARL4C and each has a nuclear localization signal and an unusually high guanine nucleotide exchange rate. This protein may play a role in membrane-associated intracellular trafficking. Mutations in this gene have been associated with Bardet-Biedl syndrome (BBS). [provided by RefSeq, Jul 2008]

Uniprot Description

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form.