SDS-Page
TBCB recombinant protein
TBCB, 1-244aa, Human, His tag, E Coli
Gene Names
TBCB; CG22; CKAP1; CKAPI
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
TBCB; 1-244aa; Human; His tag; E Coli; Tubulin folding cofactor B; CG22; CKAP1; CKAPI; TBCB recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMEVTGV SAPTVTVFIS SSLNTFRSEK RYSRSLTIAE FKCKLELLVG SPASCMELEL YGVDDKFYSK LDQEDALLGS YPVDDGCRIH VIDHSGARLG EYEDVSRVEK YTISQEAYDQ RQDTVRSFLK RSKLGRYNEE ERAQQEAEAA QRLAEEKAQA SSIPVGSRCE VRAAGQSPRR GTVMYVGLTD FKPGYWIGVR YDEPLGKNDG SVNGKRYFEC QAKYGAFVKP AVVTVGDFPE EDYGLDEI
Sequence Length
244
Applicable Applications for TBCB recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for TBCB recombinant protein
Tubulin folding cofactor B, also known as TBCB, belongs to the Microtubules family. Biosynthesis of functional microtubules involve the participation of several chaperones, termed Tubulin folding cofactors A (TBCA), B (TBCB), D (TBCD), E (TBCE) and C (TBCC), that act on folding intermediates downstream of the cytosolic chaperon, alternatively named TCP. TBCB is a 244 amino acid cytoplasmic protein containing one CAP-Gly domain and in widely expressed. TBCB is involved in the regulation of tubulin heterodimer dissociation and may function as a negative regulator of axonal growth. Recombinant human TBCB protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for TBCB recombinant protein
References
Kortazar D., et al. (2007) Exp Cell Res. 313:425-436.; Tian G., et al. (1997) J Cell Biol. 138: 821-832.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29.9 kDa (268aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
NCBI Official Full Name
tubulin-folding cofactor B isoform 1
NCBI Official Synonym Full Names
tubulin folding cofactor B
NCBI Official Symbol
TBCB
NCBI Official Synonym Symbols
CG22; CKAP1; CKAPI
NCBI Protein Information
tubulin-folding cofactor B
UniProt Protein Name
Tubulin-folding cofactor B
UniProt Gene Name
TBCB
UniProt Synonym Gene Names
CG22; CKAP1
Uniprot Description
Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer (PubMed:9265649). Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth ().
Research Articles on TBCB
Similar Products
Product Notes
The TBCB tbcb (Catalog #AAA204306) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's TBCB can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the TBCB tbcb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMEVTGV SAPTVTVFIS SSLNTFRSEK RYSRSLTIAE FKCKLELLVG SPASCMELEL YGVDDKFYSK LDQEDALLGS YPVDDGCRIH VIDHSGARLG EYEDVSRVEK YTISQEAYDQ RQDTVRSFLK RSKLGRYNEE ERAQQEAEAA QRLAEEKAQA SSIPVGSRCE VRAAGQSPRR GTVMYVGLTD FKPGYWIGVR YDEPLGKNDG SVNGKRYFEC QAKYGAFVKP AVVTVGDFPE EDYGLDEI. It is sometimes possible for the material contained within the vial of "TBCB, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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