BPNT1 recombinant protein

BPNT1, 1-308aa, Human, His tag, E Coli

Cat. Num. AAA203968

• Gene Names: BPNT1; PIP; HEL20
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: BPNT1; 1-308aa; Human; His tag; E Coli; 3' (2') 5'-bisphosphate nucleotidase 1; 3' (2'); 5'-bisphosphate nucleotidase 1; PIP; PAP-inositol-1; 4-phosphatase; BPNT1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 5mM DTT, 0.1M NaCl.
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASS NTVLMRLVAS AYSIAQKAGM IVRRVIAEGD LGIVEKTCAT DLQTKADRLA QMSICSSLAR KFPKLTIIGE EDLPSEEVDQ ELIEDSQWEE ILKQPCPSQY SAIKEEDLVV WVDPLDGTKE YTEGLLDNVT VLIGIAYEGK AIAGVINQPY YNYEAGPDAV LGRTIWGVLG LGAFGFQLKE VPAGKHIITT TRSHSNKLVT DCVAAMNPDA VLRVGGAGNK IIQLIEGKAS AYVFASPGCK KWDTCAPEVI LHAVGGKLTD IHGNVLQYHK DVKHMNSAGV LATLRNYDYY ASRVPESIKN ALVP
• Sequence Length: 308

• Applicable Applications for BPNT1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for BPNT1 recombinant protein

BPNT1, also known as BPntase, PAP phosphatase or PIP (PAP-inositol-1,4-phosphatase), is a member of the magnesium-dependent, lithium-sensitive phosphomono-esterase superfamily. using magnesium as a cofactor, BPNT1 catalyzes the conversion of PAPS (adenosine 3'-phosphate 5' phosphosulfate) to APS (adenosine 5'-phosphosulfate) and the conversion of PAP (3' (2')-phosphoadenosine 5' phosphate) to AMP (adenosine 5'-phosphate). Expressed ubiquitously with highest levels in brain and kidney, BPNT1 is potently inhibited by lithium, a drug used for the treatment of manic depression and bipolar affective disorder, suggesting a possible role for BPNT1 in the etiology of mood disorders. Recombinant human BPNT1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for BPNT1 recombinant protein

Neuroscience

References

Shaltiel G., et al. (2002) Bipolar Disord. 4 (5):302-6. ; Agam G., et al. (2003) J Psychiatr Res. 37 (5):433-42.

NCBI and Uniprot Product Information

• NCBI GI #: 116812595
• NCBI GeneID: 10380
• NCBI Accession #: NP_006076
• NCBI GenBank Nucleotide #: NM_006085.5
• UniProt Accession #: O95861; Q8WVL5; Q9UGJ3; A8K7C8; B4DPS5; B4DUS9; D3DTA9
• Molecular Weight: 37.5 kDa (344aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: 3'(2'),5'-bisphosphate nucleotidase 1 isoform 1
• NCBI Official Synonym Full Names: 3'(2'), 5'-bisphosphate nucleotidase 1
• NCBI Official Symbol: BPNT1
• NCBI Official Synonym Symbols: PIP; HEL20
• NCBI Protein Information: 3'(2'),5'-bisphosphate nucleotidase 1
• UniProt Protein Name: 3'(2'),5'-bisphosphate nucleotidase 1
• UniProt Gene Name: BPNT1
• UniProt Synonym Gene Names: PIP

NCBI Description

BPNT1, also called bisphosphate 3-prime-nucleotidase, or BPntase, is a member of a magnesium-dependent phosphomonoesterase family. Lithium, a major drug used to treat manic depression, acts as an uncompetitive inhibitor of BPntase. The predicted human protein is 92% identical to mouse BPntase. BPntase's physiologic role in nucleotide metabolism may be regulated by inositol signaling pathways. The inhibition of human BPntase may account for lithium-induced nephrotoxicity. [provided by RefSeq, Jul 2008]

Uniprot Description

Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. Has 1000-fold lower activity towards inositol 1,4-bisphosphate (Ins(1,4)P2) and inositol 1,3,4-trisphosphate (Ins(1,3,4)P3), but does not hydrolyze Ins1P, Ins(3,4)P2, Ins(1,3,4,5)P4 or InsP6.