AuH recombinant protein

AuH, 68-339aa, Human, His tag, E Coli

Cat. Num. AAA203913

• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: AuH; 68-339aa; Human; His tag; E Coli; Methylglutaconyl-CoA hydratase mitochondrial; 3 methylglutaconyl CoA hydratase; Au binding protein/enoyl CoA hydratase; Au RNA binding protein/enoyl Coenzyme A hydratase; Au specific RNA binding enoyl CoA hydratase; Methylglutaconyl CoA hydratase; Methylglutaconyl CoA hydratase mitochondrial; MGA Type I; AuH recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
• Concentration: 1.0 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSSEMKTEDE LRVRHLEEEN RGIVVLGINR AYGKNSLSKN LIKMLSKAVD ALKSDKKVRT IIIRSEVPGI FCAGADLKER AKMSSSEVGP FVSKIRAVIN DIANLPVPTI AAIDGLALGG GLELALACDI RVAASSAKMG LVETKLAIIP GGGGTQRLPR AIGMSLAKEL IFSARVLDGK EAKAVGLISH VLEQNQEGDA AYRKALDLAR EFLPQGPVAM RVAKLAINQG MEVDLVTGLA IEEACYAQTI PTKDRLEGLL AFKEKRPPRY KGE
• Sequence Length: 339

• Applicable Applications for AuH recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for AuH recombinant protein

AuH, also known as mitochondrial methylglutaconyl-CoA hydratase, is involved in the amino acid degradation pathway by catalyzing the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA and water. It has been found to have very low enoyl-CoA hydratase activity. Recombinant human AuH protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for AuH recombinant protein

Metabolism

References

Kurimoto K, et al. (2009). Proteins 75 (2):360-72; Mack M., et al. (2006). FEBS J. 273 (9):2012-22

NCBI and Uniprot Product Information

• NCBI GI #: 4502327
• NCBI GeneID: 549
• NCBI Accession #: NP_001689
• NCBI GenBank Nucleotide #: NM_001698.2
• UniProt Accession #: Q13825; Q8WUE4; B1ALV7; B1ALV8
• Molecular Weight: 31.4 kDa (293aa), confirmed by MALDI-TOF
• NCBI Official Full Name: methylglutaconyl-CoA hydratase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: AU RNA binding methylglutaconyl-CoA hydratase
• NCBI Official Symbol: AUH
• NCBI Protein Information: methylglutaconyl-CoA hydratase, mitochondrial
• UniProt Protein Name: Methylglutaconyl-CoA hydratase, mitochondrial
• Protein Family: Methylglutaconyl-CoA hydratase
• UniProt Gene Name: AUH
• UniProt Synonym Gene Names: AU-binding protein/enoyl-CoA hydratase

NCBI Description

This gene encodes bifunctional mitochondrial protein that has both RNA-binding and hydratase activities. The encoded protein is a methylglutaconyl-CoA hydratase that catalyzes the hydration of 3-methylglutaconyl-CoA to 3-hydroxy-3-methyl-glutaryl-CoA, a critical step in the leucine degradation pathway. This protein also binds AU-rich elements (AREs) found in the 3' UTRs of rapidly decaying mRNAs including c-fos, c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. This protein is localizes to the mitochondrial matrix and the inner mitochondrial membrane and may be involved in mitochondrial protein synthesis. Mutations in this gene are the cause of 3-methylglutaconic aciduria, type I. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

Catalyzes the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA (PubMed:11738050, PubMed:12434311, PubMed:12655555). Also has itaconyl-CoA hydratase activity by converting itaconyl-CoA into citramalyl-CoA in the C5-dicarboxylate catabolism pathway (PubMed:29056341). The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, a vitamin B12-poisoning metabolite (PubMed:29056341). Has very low enoyl-CoA hydratase activity (PubMed:7892223). Was originally identified as RNA-binding protein that binds in vitro to clustered 5'-AUUUA-3' motifs (PubMed:7892223).