Thymidylate synthase Recombinant Protein | TYMS recombinant protein

Thymidylate synthase, 1-313aa, Human, His tag, E Coli

Cat. Num. AAA203615

• Gene Names: TYMS; TS; TMS; HST422
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Thymidylate synthase; 1-313aa; Human; His tag; E Coli; HST422; TMS; TS; d TMP synthase; EC 2.1.1.45; MGC88736; Thymidylate synthetase; Tsase; TYMS; TYMS protein; Tyms thymidylate synthetase; ; TYMS recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1 M NaCl.
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MPVAGSELPR RPLPPAAQER DAEPRPPHGE LQYLGQIQHI LRCGVRKDDR TGTGTLSVFG MQARYSLRDE FPLLTTKRVF WKGVLEELLW FIKGSTNAKE LSSKGVKIWD ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME SDYSGQGVDQ LQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ FYVVNSELSC QLYQRSGDMG LGVPFNIASY ALLTYMIAHI TGLKPGDFIH TLGDAHIYLN HIEPLKIQLQ REPRPFPKLR ILRKVEKIDD FKAEDFQIEG YNPHPTIKME MAV
• Sequence Length: 313

• Applicable Applications for TYMS recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles

SDS-Page

Related Product Information for TYMS recombinant protein

Thymidylate synthase is an intracellular enzyme critical for de novo synthesis of DNA. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. In cancer, expression of this protein is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-Fu). Resistance or lack of response to 5-Fu is attributed to the elevation of thymidylate synthase activity. Recombinant human Thymidylate synthase protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for TYMS recombinant protein

Enzymes & Proteases

References

Chiu TH., et al. (2009) Anticancer Res. 29 (11):4503-11. ; Pena MM., et al. (2009) J Biol Chem. 284 (46):31597-607.;

NCBI and Uniprot Product Information

• NCBI GI #: 4507751
• NCBI GeneID: 7298
• NCBI Accession #: NP_001062
• NCBI GenBank Nucleotide #: NM_001071.2
• UniProt Accession #: P04818; Q8WYK3; Q8WYK4
• Molecular Weight: 37.8 kDa (333aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: thymidylate synthase
• NCBI Official Synonym Full Names: thymidylate synthetase
• NCBI Official Symbol: TYMS
• NCBI Official Synonym Symbols: TS; TMS; HST422
• NCBI Protein Information: thymidylate synthase
• UniProt Protein Name: Thymidylate synthase
• Protein Family: Thymidylate synthase
• UniProt Gene Name: TYMS
• UniProt Synonym Gene Names: TS; TS; TSase

NCBI Description

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. [provided by RefSeq, Aug 2017]

Uniprot Description

Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.