Sjogren Syndrome Antigen A1 Recombinant Protein | SSA1 recombinant protein

Recombinant Sjogren Syndrome Antigen A1 (SSA1)

Cat. Num. AAA2034548

• Gene Names: TRIM21; SSA; RO52; SSA1; RNF81; Ro/SSA
• Reactivity: Homo sapiens (Human)
• Applications: SDS-Page, Western Blot
• Purity: 97%
• Synonyms: Sjogren Syndrome Antigen A1; Recombinant Sjogren Syndrome Antigen A1 (SSA1); 52kDa; Ribonucleoprotein Autoantigen SS-A/Ro; Tripartite Motif-Containing 21; Autoantigen SSA/Ro; RING finger protein 81; SSA1 recombinant protein

• Host: E.coli
• Reactivity: Homo sapiens (Human)
• Purity/Purification: 97%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
• Concentration: Original Concentration: 200ug/mL (varies by lot)

• Applicable Applications for SSA1 recombinant protein: Positive Control; Immunogen; SDS-PAGE (SDS); Western Blot (WB)
• Application Notes: May be suitable for use in other assays to be determined by the end user.
• Source: Prokaryotic expression
• Residues: Glu268~Leu465
• Tags: N-terminal His Tag
• Subcellular Location: Nucleus, Cytoplasm
• Traits: Freeze-dried powder
• Predicted isoelectric point: 6.8
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8ºC for one month.; Aliquot and store at -80ºC for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence

Testing Data

(Identification)

SDS-PAGE (SDS)

NCBI and Uniprot Product Information

• NCBI GI #: 53690156
• NCBI GeneID: 6737
• Molecular Weight: Predicted Molecular Mass: 26.1kDa; Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: Sjogren syndrome antigen A1
• NCBI Official Synonym Full Names: tripartite motif containing 21
• NCBI Official Symbol: TRIM21
• NCBI Official Synonym Symbols: SSA; RO52; SSA1; RNF81; Ro/SSA
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM21
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM21
• Protein Family: Serotype-specific antigen
• UniProt Gene Name: TRIM21
• UniProt Synonym Gene Names: RNF81; RO52; SSA1; SS-A
• UniProt Entry Name: RO52_HUMAN

NCBI Description

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM21: E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)- like complex is shown to mediate ubiquitination of CDKN1B ('Thr- 187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin- mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytoplasm; cytosol; nucleus; ribonucleoprotein complex; SCF ubiquitin ligase complex

Molecular Function: identical protein binding; protein binding; ubiquitin-protein ligase activity

Biological Process: inhibition of NF-kappaB transcription factor; innate immune response; negative regulation of viral transcription; positive regulation of autophagy; positive regulation of cell cycle; positive regulation of transcription factor activity; protein autoubiquitination; protein destabilization; protein monoubiquitination; protein polyubiquitination; protein ubiquitination; regulation of interferon type I production