Src Kinase Associated Phosphoprotein 1 Recombinant Protein | SKAP1 recombinant protein

Recombinant Src Kinase Associated Phosphoprotein 1 (SKAP1)

Cat. Num. AAA2034418

• Gene Names: SKAP1; SCAP1; SKAP55; HEL-S-81p
• Reactivity: Mouse
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Src Kinase Associated Phosphoprotein 1; Recombinant Src Kinase Associated Phosphoprotein 1 (SKAP1); SCAP1; SKAP55; pp55; Src Family Associated Phosphoprotein 1; Src kinase-associated phosphoprotein of 55 kDa; SKAP1 recombinant protein

• Host: E Coli
• Reactivity: Mouse
• Purity/Purification: >90%
• Sequence Length: 359

• Applicable Applications for SKAP1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Fragment: Met1~Ile355
• Tag: Two N-terminal Tags, His-tag and T7-tag
• Expression System: Prokaryotic Expression
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 115527074
• NCBI GeneID: 8631
• NCBI Accession #: NP_003717.3
• NCBI GenBank Nucleotide #: NM_003726.3
• Molecular Weight: 44.7kDa
• NCBI Official Full Name: src kinase-associated phosphoprotein 1 isoform 1
• NCBI Official Synonym Full Names: src kinase associated phosphoprotein 1
• NCBI Official Symbol: SKAP1
• NCBI Official Synonym Symbols: SCAP1; SKAP55; HEL-S-81p
• NCBI Protein Information: src kinase-associated phosphoprotein 1
• UniProt Protein Name: Src kinase-associated phosphoprotein 1
• Protein Family: Src kinase-associated phosphoprotein
• UniProt Gene Name: SKAP1
• UniProt Synonym Gene Names: SCAP1; SKAP55; SKAP-55; pp55
• UniProt Entry Name: SKAP1_HUMAN

NCBI Description

This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins. [provided by RefSeq, Jul 2008]

Uniprot Description

SKAP55: Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells. Belongs to the SKAP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: cytoplasm; cytosol; intercellular junction; nucleus; plasma membrane; T cell receptor complex

Molecular Function: protein binding; protein complex binding; protein kinase binding; protein phosphatase binding; SH2 domain binding; SH3 domain binding; SH3/SH2 adaptor activity

Biological Process: adaptive immune response; positive regulation of cell adhesion; positive regulation of signal transduction; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; T cell receptor signaling pathway