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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Mouse Inter Alpha-Globulin Inhibitor H4 Polyclonal Antibody | anti-ITIH4 antibody

Polyclonal Antibody to Inter Alpha-Globulin Inhibitor H4 (ITIH4)

Gene Names
Itih4; Itih-4; PK-120; ITI-HC4
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Inter Alpha-Globulin Inhibitor H4; Polyclonal Antibody; Polyclonal Antibody to Inter Alpha-Globulin Inhibitor H4 (ITIH4); IHRP; PK120; H4P; ITIHL1; Plasma Kallikrein-Sensitive Glycoprotein; Plasma kallikrein sensitive glycoprotein 120; Inter-alpha-trypsin inhibitor family heavy chain-related protein; anti-ITIH4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ITIH4. It has been selected for its ability to recognize ITIH4 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
157
Applicable Applications for anti-ITIH4 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Fragment
ITIH4 (His721~Ile942)
Conjugate
No Conjugate
Cross Reactivity
Mouse
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant ITIH4 (His721~Ile942) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2080338
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded liver tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded liver tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Liver Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Liver Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
100,395 Da
NCBI Official Full Name
inter alpha-trypsin inhibitor, heavy chain 4 isoform 2
NCBI Official Synonym Full Names
inter alpha-trypsin inhibitor, heavy chain 4
NCBI Official Symbol
Itih4
NCBI Official Synonym Symbols
Itih-4; PK-120; ITI-HC4
NCBI Protein Information
inter alpha-trypsin inhibitor, heavy chain 4
UniProt Protein Name
Inter alpha-trypsin inhibitor, heavy chain 4
UniProt Gene Name
Itih4
UniProt Synonym Gene Names
ITI heavy chain H4; ITI-HC4; Inter-alpha-inhibitor heavy chain 4

NCBI Description

This gene encodes a member of the inter-alpha trypsin inhibitor (IaI) family of plasma serine protease inhibitors with diverse functions as anti-apoptotic and matrix stabilizing molecules during development. This gene is predominantly expressed in the liver and the encoded protein was found to be a plasma kallikrein-sensitive glycoprotein. This gene is located in a cluster of related inter alpha trypsin inhibitor genes on chromosome 14. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]

Uniprot Description

ITIH4: May be involved in acute phase reactions. Belongs to the ITIH family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14 B|14 19.09 cM

Cellular Component: cytoplasm; extracellular region; plasma membrane

Molecular Function: calcium ion binding; hyaluronic acid binding; peptidase inhibitor activity; protein binding; serine-type endopeptidase inhibitor activity

Biological Process: acute-phase response; hyaluronan metabolic process; negative regulation of peptidase activity

Research Articles on ITIH4

Similar Products

Product Notes

The ITIH4 itih4 (Catalog #AAA2033955) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Inter Alpha-Globulin Inhibitor H4 (ITIH4) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Inter Alpha-Globulin Inhibitor H4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the ITIH4 itih4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Inter Alpha-Globulin Inhibitor H4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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