NDRG1 active protein

NDRG1, 1-394aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA203387

• Gene Names: NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: NDRG1; 1-394aa; Human; His tag; E Coli (Bioactivity Validated); N-myc downstream regulated 1; CAP43; CMT4D; DRG1; GC4; HMSNL; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5; N-myc downstream regulated 1 TDD5; 42 kDa; anti-GC4; cmt4d; Differentiation related gene1 protein; Drg 1; hmsnl; Human mRNA for RTP complete cds; tdds; N myc downstream regulated gene 1; N myc downstream regulated gene 1 protein; Ndr 1; ndr1; NDRG 1; NDRG1 protein; Protein nickel specific induction protein; Nickel specific induction protein Cap43; nmsl; Nmyc downstream regulated; reduced in tumor; reducin; Nmyc downstream regulated gene1; Nmyc downstream regulated gene1 protein; Protein regulated by oxygen 1; Protein regulated by oxygen1; proxy1; Reducing agents and tunicamycin responsive protein; tunicamycin-responsive protein; NDRG1 active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.1 mM PMSF, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MSREMQDVDL AEVKPLVEKG ETITGLLQEF DVQEQDIETL HGSVHVTLCG TPKGNRPVIL TYHDIGMNHK TCYNPLFNYE DMQEITQHFA VCHVDAPGQQ DGAASFPAGY MYPSMDQLAE MLPGVLQQFG LKSIIGMGTG AGAYILTRFA LNNPEMVEGL VLINVNPCAE GWMDWAASKI SGWTQALPDM VVSHLFGKEE MQSNVEVVHT YRQHIVNDMN PGNLHLFINA YNSRRDLEIE RPMPGTHTVT LQCPALLVVG DSSPAVDAVV ECNSKLDPTK TTLLKMADCG GLPQISQPAK LAEAFKYFVQ GMGYMPSASM TRLMRSRTAS GSSVTSLDGT RSRSHTSEGT RSRSHTSEGT RSRSHTSEGA HLDITPNSGA AGNSAGPKSM EVSCLEHHHH HH
• Sequence Length: 394

• Applicable Applications for NDRG1 active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: The ED50 for this effect is 0.5 - 1.5 ng/ml. Measured in a cell proliferation assay using MCF7 cell.
• Assay: 1. Cell line: MCF7 (Human breast adenocarcinoma cell); 2. Maintenance Condition: RPMI 1640 containing 10% FBS; 3. Assay medium: serum free RPMI 1640; 4. Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul); 5. Incubation time : 40 hrs (after sample treatment); 6. Concentration range: 0.19ng/ml - 50ng/ml; 7. Detection method : Brdu assay
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for NDRG1 active protein

N-myc downstream regulated gene (NDRG)-1 is one of 4 members of the NDRG alpha/beta-hydrolase family. This protein is classified in databases as a tumor suppressor and heavy metal-response protein. Its functions include cell-cycle regulation, cellular differentiation, apoptosis, hypoxia response and metal-ion sensing. Mutation in the NDRG1 gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom (HMSNL), a severe autosomal recessive form of Charcot-Marie-Tooth (CMT) disease. Recombinant human NDRG1, fused to His-tag at C-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for NDRG1 active protein

Cancer

References

Richardson DR., et al. (2008) Biochim Biophys Acta. 1783 (10):1981-92.; Costa M., et al. (2008) Carcinogenesis. 29 (1):2-8.;

NCBI and Uniprot Product Information

• NCBI GI #: 37655183
• NCBI GeneID: 10397
• NCBI Accession #: NP_006087
• NCBI GenBank Nucleotide #: NM_006096.3
• UniProt Accession #: Q92597; O15207; Q6IBG2; Q9NYR6; Q9UK29; B3KR80; B7Z446
• Molecular Weight: 43.9 kDa (402aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: protein NDRG1 isoform 1
• NCBI Official Synonym Full Names: N-myc downstream regulated 1
• NCBI Official Symbol: NDRG1
• NCBI Official Synonym Symbols: GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1
• NCBI Protein Information: protein NDRG1
• UniProt Protein Name: Protein NDRG1
• Protein Family: Protein
• UniProt Gene Name: NDRG1
• UniProt Synonym Gene Names: CAP43; DRG1; RTP; DRG-1; RTP

NCBI Description

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy.