Mediator Complex Subunit 1 Recombinant Protein | MED1 recombinant protein

Recombinant Mediator Complex Subunit 1 (MED1)

Cat. Num. AAA2033638

• Gene Names: MED1; PBP; CRSP1; RB18A; TRIP2; PPARBP; CRSP200; DRIP205; DRIP230; PPARGBP; TRAP220
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Mediator Complex Subunit 1; Recombinant Mediator Complex Subunit 1 (MED1); PPARBP; ARC205; PBP; CRSP1; CRSP200; PPARGBP; RB18A; TRAP220; TRIP2; Peroxisome proliferator-activated receptor-binding protein; Thyroid receptor-interacting protein 2; MED1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: MKAQGETEES EKLSKMSSLL ERLHAKFNQN RPWSETIKLV RQVMEKRVVM SSGGHQHLVS CLETLQKALK VTSLPAMTDR LESIARQNGL GSHLSASGTE CYITSDMFYV EVQLDPAGQL CDVKVAHHGE NPVSCPELVQ QLREKNFDEF SKHLKGLVNL YNLPGDNKLK TKMYLALQSLEQDLSKMAIM YWKATNAGPL DKILHGSVGY LT
• Sequence Length: 1581

• Applicable Applications for MED1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 27.5kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Thr212
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 124298020
• NCBI GeneID: 5469
• Molecular Weight: 61,563 Da
• NCBI Official Full Name: Mediator complex subunit 1
• NCBI Official Synonym Full Names: mediator complex subunit 1
• NCBI Official Symbol: MED1
• NCBI Official Synonym Symbols: PBP; CRSP1; RB18A; TRIP2; PPARBP; CRSP200; DRIP205; DRIP230; PPARGBP; TRAP220
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 1
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 1
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED1
• UniProt Synonym Gene Names: ARC205; CRSP1; CRSP200; DRIP205; DRIP230; PBP; PPARBP; PPARGBP; RB18A; TRAP220; TRIP2; ARC205; PBP; PPAR-binding protein; Trap220; TR-interacting protein 2; TRIP-2
• UniProt Entry Name: MED1_HUMAN

NCBI Description

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008]

Uniprot Description

MED1: a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. Also is a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. May regulates p53-dependent apoptosis. Is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: chromatin; membrane; nucleolus; nucleoplasm; nucleus; Srb-mediator complex; ubiquitin ligase complex

Molecular Function: chromatin binding; chromatin DNA binding; estrogen receptor binding; LBD domain binding; ligand-dependent nuclear receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; nuclear hormone receptor binding; peroxisome proliferator activated receptor binding; protein binding; receptor activity; retinoic acid receptor binding; thyroid hormone receptor binding; thyroid hormone receptor coactivator activity; transcription coactivator activity; transcription cofactor activity; transcription factor binding; vitamin D receptor binding

Biological Process: androgen biosynthetic process; androgen receptor signaling pathway; angiogenesis; brain development; cell morphogenesis; cellular lipid metabolic process; embryonic heart tube development; embryonic hemopoiesis; embryonic hindlimb morphogenesis; embryonic placenta development; enucleate erythrocyte development; erythrocyte development; fat cell differentiation; gene expression; keratinocyte differentiation; lactation; lens development in camera-type eye; liver development; monocyte differentiation; mRNA transcription from RNA polymerase II promoter; negative regulation of apoptosis; negative regulation of neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; organ regeneration; positive regulation of erythrocyte differentiation; positive regulation of estrogen receptor signaling pathway; positive regulation of keratinocyte differentiation; positive regulation of mammary gland epithelial cell proliferation; positive regulation of protein import into nucleus, translocation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein ubiquitination; regulation of cell cycle; regulation of transcription from RNA polymerase I promoter; steroid hormone receptor signaling pathway; thyroid hormone generation; transcription initiation from RNA polymerase II promoter