Constitutive Androstane Receptor Recombinant Protein | CAR recombinant protein

Recombinant Constitutive Androstane Receptor (CAR)

Cat. Num. AAA2033468

• Gene Names: NR1I3; CAR; CAR1; MB67
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Constitutive Androstane Receptor; Recombinant Constitutive Androstane Receptor (CAR); NR1I3; NR1-I3; CAR1; MB67; Nuclear Receptor Subfamily 1; Group I; Member 3; Constitutive active response; Constitutive activator of retinoid response; Orphan nuclear receptor MB67; CAR recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: MTATLTLETM TSEEEYGPRN CVVCGDRATG YHFHALTCEG CKGFFRRTVS KTIGPICPFA GRCEVSKAQR RHCPACRLQK CLNVGMRKDM ILSAEALALR RARQARRRAQ KASLQLSQQQ KELIQTLLGA HTRHVGPMFD QFVQFRPPAY LFSHHRPFQP LAPVLPLLTH FADINTFMVQ QIIKFTKDLP LFRSLTMEDQ ISLLKGAAVE ILHISLNTTF CLQTQNFFCGPLCYKMEDAV HVGFQYE
• Sequence Length: 309

• Applicable Applications for CAR recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 31.8kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Glu247
• Organism Species: Rat
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 216409712
• NCBI GeneID: 9970
• Molecular Weight: 30,262 Da
• NCBI Official Full Name: constitutive androstane receptor
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1 group I member 3
• NCBI Official Symbol: NR1I3
• NCBI Official Synonym Symbols: CAR; CAR1; MB67
• NCBI Protein Information: nuclear receptor subfamily 1 group I member 3
• UniProt Protein Name: Nuclear receptor subfamily 1 group I member 3
• Protein Family: Carboxylic acid reductase
• UniProt Gene Name: NR1I3
• UniProt Synonym Gene Names: CAR; Constitutive active response; CAR
• UniProt Entry Name: NR1I3_HUMAN

NCBI Description

This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NR1I3: Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element. Interacts with ECT2. Heterodimer of NR1I3 and RXR. Interacts with PSMC4. Directly interacts with DNAJC7. The DNAJC7-NR1I3 complex may also include HSP90. By dexamethasone. Predominantly expressed in liver. Belongs to the nuclear hormone receptor family. NR1 subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: cytoplasm; cytoskeleton; cytosol; nucleoplasm; nucleus

Molecular Function: androgen receptor activity; DNA binding; ligand-dependent nuclear receptor activity; thyroid hormone receptor activity; transcription coactivator activity; transcription factor activity; zinc ion binding

Biological Process: androgen receptor signaling pathway; gene expression; intracellular receptor-mediated signaling pathway; negative regulation of transcription, DNA-dependent; osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; signal transduction; steroid hormone mediated signaling; transcription initiation from RNA polymerase II promoter