Guanylate Binding Protein 4 Recombinant Protein | GBP4 recombinant protein

Recombinant Guanylate Binding Protein 4 (GBP4)

Cat. Num. AAA2033195

• Gene Names: GBP4; Mpa2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Guanylate Binding Protein 4; Recombinant Guanylate Binding Protein 4 (GBP4); GTP-binding protein 4; Guanine nucleotide-binding protein 4; GBP4 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: MEAPICLVEN WKNQLTVNLE AIRILEQIAQ PLVVVAIVGL YRTGKSYLMN RLAGRNHGFS LGSTVQSETK GIWMWCVPHP TKPTHTLVLL DTEGLGDVEK GDPKNDSWIF ALAVLLSSTF VYNSMSTINQ QALEQLHFVT ELTQLIRAKS SPREDKVKDS SEFVGFFPDF IWAVRDFALE LKLNGRPITE DEYLENALKL IQGDNLKVQQ SNMTRECIRY FFPVRKCFVF DRPTSDKRLL LQIENVPENQ LERNFQVESE KFCSYIFTNG KTKTLRGGVI VTGNRLGTLV QTYVNAINSGTVPC
• Sequence Length: 640

• Applicable Applications for GBP4 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 38.2kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Cys304
• Organism Species: Mouse
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 31377634
• NCBI GeneID: 115361
• NCBI Accession #: NP_443173.2
• NCBI GenBank Nucleotide #: NM_052941.4
• Molecular Weight: 73,165 Da
• NCBI Official Full Name: guanylate-binding protein 4
• NCBI Official Synonym Full Names: guanylate binding protein 4
• NCBI Official Symbol: GBP4
• NCBI Official Synonym Symbols: Mpa2
• NCBI Protein Information: guanylate-binding protein 4
• UniProt Protein Name: Guanylate-binding protein 4
• Protein Family: Guanylate-binding protein
• UniProt Gene Name: GBP4
• UniProt Synonym Gene Names: GBP-4
• UniProt Entry Name: GBP4_HUMAN

NCBI Description

Guanylate-binding proteins, such as GBP4, are induced by interferon and hydrolyze GTP to both GDP and GMP (Vestal, 2005 [PubMed 16108726]).[supplied by OMIM, Dec 2008]

Uniprot Description

GBP4: Binds GTP, GDP and GMP. Hydrolyzes GTP very efficiently; GDP rather than GMP is the major reaction product. Plays a role in erythroid differentiation. Belongs to the GBP family.

Protein type: Cell adhesion; Hydrolase; Vesicle

Chromosomal Location of Human Ortholog: 1p22.2

Cellular Component: Golgi membrane; nucleus; perinuclear region of cytoplasm

Molecular Function: GTP binding; GTPase activity

Biological Process: metabolic process