Cyclic ADP Ribose Hydrolase Recombinant Protein | cADPRH recombinant protein

Recombinant Cyclic ADP Ribose Hydrolase (cADPRH)

Cat. Num. AAA2033153

• Gene Names: CD38; ADPRC1; ADPRC 1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cyclic ADP Ribose Hydrolase; Recombinant Cyclic ADP Ribose Hydrolase (cADPRH); CD38; ADPRC 1; c-ADPRH; T10; p45; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; cADPRH recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: DCQSVWDAFK GAFISKHPCNITEEDYQPLM KLGTQTVPCN KILLWSRIKD LAHQFTQVQR DMFTLEDTLLGYLADDLTWC GEFNTSKINY QSCPDWRKDC SNNPVSVFWK TVSRRFAEAACDVVHVMLNG SRSKIFDKNS TFGSVEVHNL QPEKVQTLEA WVIHGGREDSRDLCQDPTIK ELESIISKRN IQFSCKNIYR PDKFLQCVKN PEDSSCTS
• Sequence Length: 60

• Applicable Applications for cADPRH recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 28.8kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Asp81~Ser298
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 68989438
• NCBI GeneID: 952
• Molecular Weight: 13,788 Da
• NCBI Official Full Name: cyclic ADP-ribose hydrolase, partial
• NCBI Official Synonym Full Names: CD38 molecule
• NCBI Official Symbol: CD38
• NCBI Official Synonym Symbols: ADPRC1; ADPRC 1
• NCBI Protein Information: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• UniProt Protein Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• UniProt Gene Name: CD38
• UniProt Synonym Gene Names: ADPRC 1; cADPr hydrolase 1
• UniProt Entry Name: CD38_HUMAN

NCBI Description

The protein encoded by this gene is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

CD38: Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. Belongs to the ADP-ribosyl cyclase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.2.6; Cell cycle regulation; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Membrane protein, integral; Apoptosis; Cell surface; Lyase; Hydrolase; EC 2.4.99.20

Chromosomal Location of Human Ortholog: 4p15

Cellular Component: cell surface; cytosol; integral to membrane; membrane; nucleus; plasma membrane

Molecular Function: NAD(P)+ nucleosidase activity; NAD+ nucleosidase activity; phosphorus-oxygen lyase activity; transferase activity

Biological Process: B cell receptor signaling pathway; elevation of cytosolic calcium ion concentration; female pregnancy; metabolic process; negative regulation of apoptosis; negative regulation of bone resorption; negative regulation of transcription, DNA-dependent; positive regulation of B cell proliferation; positive regulation of cell growth; positive regulation of insulin secretion; positive regulation of transcription, DNA-dependent; positive regulation of vasoconstriction; response to drug; response to estradiol stimulus; response to hydroperoxide; response to hypoxia; response to progesterone stimulus; response to retinoic acid; signal transduction