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Western Blot (WB) (Western blot analysis of VTI1A Antibody (C-term) in mouse spleen tissue lysates (35ug/lane). VTI1A (arrow) was detected using the purified Pab.)

Rabbit anti-Human, Mouse Vesicle transport through interaction with t-SNAREs homolog 1A Polyclonal Antibody | anti-Vti1a antibody

Vesicle transport through interaction with t-SNAREs homolog 1A, CT (MVti1, Vesicle transport through interaction with t-SNAREs homolog 1A, Vesicle transport v-SNARE protein Vti1-like 2, Vti1a, Vti1-rp2) (APC)

Gene Names
VTI1A; MVti1; VTI1RP2; Vti1-rp2
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
Vesicle transport through interaction with t-SNAREs homolog 1A; Polyclonal Antibody; CT (MVti1; Vesicle transport v-SNARE protein Vti1-like 2; Vti1a; Vti1-rp2) (APC); anti-Vti1a antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human VTI1A. Species Crossreactivity: mouse.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-Vti1a antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-1:500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human VTI1A (KLH).
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of VTI1A Antibody (C-term) in mouse spleen tissue lysates (35ug/lane). VTI1A (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of VTI1A Antibody (C-term) in mouse spleen tissue lysates (35ug/lane). VTI1A (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma with VTI1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lung carcinoma with VTI1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NCI-H292 cells using VTI1A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of NCI-H292 cells using VTI1A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of VTI1A (arrow) using rabbit polyclonal VTI1A Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VTI1A gene.)

Western Blot (WB) (Western blot analysis of VTI1A (arrow) using rabbit polyclonal VTI1A Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VTI1A gene.)
Product Categories/Family for anti-Vti1a antibody
References
1. Tai, G., et.al., Mol. Biol. Cell 15(9), 4011-4022 (2004). 2. Mallard, F., et.al., J. Cell Biol. 156(4), 653-664 (2002).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,218 Da
NCBI Official Full Name
vesicle transport through interaction with t-SNAREs homolog 1A
NCBI Official Synonym Full Names
vesicle transport through interaction with t-SNAREs 1A
NCBI Official Symbol
VTI1A
NCBI Official Synonym Symbols
MVti1; VTI1RP2; Vti1-rp2
NCBI Protein Information
vesicle transport through interaction with t-SNAREs homolog 1A; SNARE Vti1a-beta protein; vesicle transport v-SNARE protein Vti1-like 2
UniProt Protein Name
Vesicle transport through interaction with t-SNAREs homolog 1A
UniProt Gene Name
VTI1A
UniProt Entry Name
VTI1A_HUMAN

Uniprot Description

VTI1A: V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non- conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be involved in increased cytokine secretion associated with cellular senescence. Belongs to the VTI1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q25.2

Cellular Component: SNARE complex; Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; synaptic vesicle; Golgi stack; clathrin-coated vesicle; cell soma; perinuclear region of cytoplasm; late endosome membrane; integral to membrane; endosome

Molecular Function: SNAP receptor activity; SNARE binding

Biological Process: intra-Golgi vesicle-mediated transport; voluntary musculoskeletal movement; ER to Golgi vesicle-mediated transport; Golgi to vacuole transport; protein targeting to vacuole; vesicle fusion with Golgi apparatus; retrograde transport, endosome to Golgi

Disease: Multiple Mitochondrial Dysfunctions Syndrome 3

Research Articles on Vti1a

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Product Notes

The Vti1a vti1a (Catalog #AAA6505729) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Vesicle transport through interaction with t-SNAREs homolog 1A, CT (MVti1, Vesicle transport through interaction with t-SNAREs homolog 1A, Vesicle transport v-SNARE protein Vti1-like 2, Vti1a, Vti1-rp2) (APC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Vesicle transport through interaction with t-SNAREs homolog 1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-1:500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Vti1a vti1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vesicle transport through interaction with t-SNAREs homolog 1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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