Transcriptional Regulating Factor 1 Recombinant Protein | TRERF1 recombinant protein

Recombinant Transcriptional Regulating Factor 1 (TRERF1)

Cat. Num. AAA2033074

• Gene Names: TRERF1; RAPA; BCAR2; TREP132; TReP-132; HSA277276; dJ139D8.5
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Transcriptional Regulating Factor 1; Recombinant Transcriptional Regulating Factor 1 (TRERF1); BCAR2; TREP132; RAPA; Breast Cancer Anti-Estrogen Resistance 2; Transcriptional-regulating protein 132; Zinc finger protein rapa; Zinc finger transcription factor TReP-132; TRERF1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: PR INIGLRFQAE IPELQDISAL AQDTHKATLV WKPWPELENH DLQQRVENLL NLCCSSALPG GGTNSEFALH SLFEAKGDVM VALEMLLLRK PVRLKCHPLA NYHYAGSDKW TSLERKLFNKALATYSKDFI FVQKMVKSKT VAQCVEYYYT WKKIMR
• Sequence Length: 1220

• Applicable Applications for TRERF1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 21.9kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Pro779~Arg936
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 662033858
• NCBI GeneID: 55809
• NCBI Accession #: NP_001284502.1
• NCBI GenBank Nucleotide #: NM_001297573.1
• Molecular Weight: 124,999 Da
• NCBI Official Full Name: transcriptional-regulating factor 1 isoform 1
• NCBI Official Synonym Full Names: transcriptional regulating factor 1
• NCBI Official Symbol: TRERF1
• NCBI Official Synonym Symbols: RAPA; BCAR2; TREP132; TReP-132; HSA277276; dJ139D8.5
• NCBI Protein Information: transcriptional-regulating factor 1
• UniProt Protein Name: Transcriptional-regulating factor 1
• Protein Family: Transcriptional-regulating factor
• UniProt Gene Name: TRERF1
• UniProt Synonym Gene Names: BCAR2; RAPA; TREP132
• UniProt Entry Name: TREF1_HUMAN

NCBI Description

This gene encodes a zinc-finger transcriptional regulating protein which interacts with CBP/p300 to regulate the human gene CYP11A1. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]

Uniprot Description

TReP-132: Binds DNA and activates transcription of CYP11A1. Interaction with CREBBP and EP300 results in a synergistic transcriptional activation of CYP11A1

Protein type: C2H2-type zinc finger protein; Nuclear receptor co-regulator; Transcription factor

Chromosomal Location of Human Ortholog: 6p21.1-p12.1

Cellular Component: histone deacetylase complex; nucleus; transcription factor complex

Molecular Function: transcription factor activity; transcription factor binding

Biological Process: positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter