Western Blot (WB)
(Western Blot:Sample: Recombinant ZRF1, Human.)
Rabbit Zuotin Related Factor 1 Polyclonal Antibody | anti-ZRF1 antibody
Zuotin Related Factor 1 (ZRF1) Polyclonal Antibody
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot:Sample: Recombinant ZRF1, Human.)
Western Blot (WB)
(Western Blot:Sample: Recombinant ZRF1, Human.)
Western Blot (WB)
(Western Blot;Sample:Lane 1: Mouse Testis lysate;Lane 2: Human Hela cell lysate;Lane 3: Human 293T cell lysate;Lane 4: Human Jurkat cell lysatePrimary Ab: 1ug/mL Rabbit Anti-Human ZRF1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Western Blot (WB)
(Western Blot;Sample:Lane 1: Mouse Testis lysate;Lane 2: Human Hela cell lysate;Lane 3: Human 293T cell lysate;Lane 4: Human Jurkat cell lysatePrimary Ab: 1ug/mL Rabbit Anti-Human ZRF1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Colon Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human ZRF1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Colon Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human ZRF1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the M-phase phosphoprotein (MPP) family. The gene encodes a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. The protein is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. This protein was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Also, chromosomal aberrations involving this gene are associated with primary head and neck squamous cell tumors. This gene has a pseudogene on chromosome 6. Alternatively spliced variants which encode different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
DNAJC2: Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb- repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell cycle regulation; Chaperone; DNA replication
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: cytoplasm; cytosol; nuclear membrane; nucleoplasm; nucleus
Molecular Function: ATPase activator activity; chromatin binding; DNA binding; histone binding; Hsp70 protein binding; RNA binding
Biological Process: covalent chromatin modification; de novo'cotranslational protein folding; DNA replication; positive regulation of ATPase activity; positive regulation of transcription, DNA-templated; transcription, DNA-dependent
Research Articles on ZRF1
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Product Notes
The ZRF1 dnajc2 (Catalog #AAA2032908) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Zuotin Related Factor 1 (ZRF1) Polyclonal Antibody reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Zuotin Related Factor 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the ZRF1 dnajc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Zuotin Related Factor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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