Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Liver Tissue; Lane2: Mouse Testis Tissue; Lane3: Mouse Brain Tissue.)
Rabbit anti-Mouse Guanidinoacetate-N-Methyltransferase Polyclonal Antibody | anti-GAMT antibody
Guanidinoacetate-N-Methyltransferase (GAMT) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Liver Tissue; Lane2: Mouse Testis Tissue; Lane3: Mouse Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Liver Tissue; Lane2: Mouse Testis Tissue; Lane3: Mouse Brain Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant GAMT, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant GAMT, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Uterus Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Uterus Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Testis Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Testis Tissue)
NCBI and Uniprot Product Information
Uniprot Description
GAMT: Defects in GAMT are the cause of guanidinoacetate methyltransferase deficiency (GAMT deficiency). GAMT deficiency is an autosomal recessive disorder characterized by developmental delay/regression, mental retardation, severe disturbance of expressive and cognitive speech, intractable seizures and movement disturbances, severe depletion of creatine/phosphocreatine in the brain, and accumulation of guanidinoacetic acid (GAA) in brain and body fluids. Belongs to the class I-like SAM-binding methyltransferase superfamily. RMT2 methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - glycine, serine and threonine; Contractile; EC 2.1.1.2; Methyltransferase
Chromosomal Location of Human Ortholog: 10 C1|10 39.72 cM
Cellular Component: cell projection; cytoplasm; nucleus
Molecular Function: guanidinoacetate N-methyltransferase activity; methyltransferase activity; protein homodimerization activity; S-adenosylmethionine-dependent methyltransferase activity; transferase activity
Biological Process: creatine biosynthetic process; methylation; organ morphogenesis; regulation of multicellular organism growth; S-adenosylhomocysteine metabolic process; S-adenosylmethionine metabolic process; spermatogenesis
Research Articles on GAMT
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Product Notes
The GAMT gamt (Catalog #AAA2032462) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Guanidinoacetate-N-Methyltransferase (GAMT) Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Guanidinoacetate-N-Methyltransferase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the GAMT gamt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Guanidinoacetate-N-Methyltransferase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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