Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Rabbit anti-Rat Ectonucleoside Triphosphate Diphosphohydrolase 1 Polyclonal Antibody | anti-ENTPD1 antibody
Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Liver Tissue)
NCBI and Uniprot Product Information
NCBI Description
has apyrase activity; may inactivate signaling mediated by extracellular nucleotides [RGD, Feb 2006]
Uniprot Description
ENTPD1: an ectonucleotidase that, like CD73, hydrolyzes immunostimulatory ATP, and ADP, to immunosuppressive AMP. May inhibit host immune responses against cancer. Expressed at high levels in malignant epithelial cells of human rectal adenocarcinoma. A prognostic marker of chronic lymphocytic leukemia that may identify patients with an unfavorable clinical outcome. Synergizes with CD161 to modulate Th17 responses in Crohn's disease. It may hydrolyze ATP and other nucleotides in the nervous system to regulate purinergic neurotransmission in the nervous system. May be implicated in inhibiting platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. Belongs to the GDA1/CD39 NTPase family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.6.1.5; Extracellular matrix; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine
Chromosomal Location of Human Ortholog: 1q54
Cellular Component: basal lamina; basolateral plasma membrane; cell soma; cell surface; extracellular space; integral component of membrane; membrane; neuron projection; postsynaptic density; synaptic vesicle
Molecular Function: adenosine-diphosphatase activity; ATP binding; ATPase activity; hydrolase activity; identical protein binding; nucleoside-diphosphatase activity; nucleoside-triphosphatase activity
Biological Process: G-protein coupled receptor signaling pathway; negative regulation of dopamine secretion; platelet activation; protein homooligomerization; purine ribonucleoside diphosphate catabolic process; response to ATP; response to caffeine; response to gamma radiation; response to lipopolysaccharide; response to morphine; response to proline
Research Articles on ENTPD1
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Product Notes
The ENTPD1 entpd1 (Catalog #AAA2032447) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ectonucleoside Triphosphate Diphosphohydrolase 1 (ENTPD1) Polyclonal Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ectonucleoside Triphosphate Diphosphohydrolase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ENTPD1 entpd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ectonucleoside Triphosphate Diphosphohydrolase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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