Cyclophilin H Active Protein | PPIH active protein

Cyclophilin H (PPIH), 1-177aa, Human,E Coli (Bioactivity Validated)

Cat. Num. AAA203201

• Gene Names: PPIH; CYPH; CYP-20; USA-CYP; SnuCyp-20
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Cyclophilin H; Cyclophilin H (PPIH); 1-177aa; Human; E Coli (Bioactivity Validated); Peptidylprolyl isomerase H; PPIH; CYPH; CYP20; SnuCyp-20; PPIase H; Rotamase H; Peptidylprolyl isomerase H CypH; EC 5.2.1.8; MGC5016; Small nuclear ribonucleoprotein particle-specific cyclophilin H; uSACYP; usnRNP associated cyclophilin SnuCyp20; ; PPIH active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid in phosphate buffered saline (pH 7.4), 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MAVANSSPVN PVVFFDVSIG GQEVGRMKIE LFADVVPKTA ENFRQFCTGE FRKDGVPIGY KGSTFHRVIK DFMIQGGDFV NGDGTGVASI YRGPFADENF KLRHSAPGLL SMANSGPSTN GCQFFITCSK CDWLDGKHVV FGKIIDGLLV MRKIENVPTG PNNKPKLPVV ISQCGEM
• Sequence Length: 177

• Applicable Applications for PPIH active protein: SDS-PAGE
• Antigen Species: Human
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.
• Assay: 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.; 2. Add 10ul of recombinant Cyclophikin H (PPIH) protein with 1ug in assay buffer.; 3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer.; 4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM); 5. Record the increase in A405 nm for 30 minutes at 25C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PPIH active protein

Cyclophilin H (also known as peptidylpropyl isomerase H, PPIH) is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. The cyclophilin H is a specific component of the human u4/u6 small nuclear ribonucleoprotein particle involved in the nuclear splicing of pre-mRNA. It stably associates with the u4/u6-60kD and -90kD proteins, the human orthologues of the Saccharomyces cerevisiae Prp4 and Prp3 splicing factors. Recombinant human cyclophilin H was expressed in E Coli and purified by conventional chromatography techniques.

Product Categories/Family for PPIH active protein

Cell Cycle

References

Reidt u., et al. (2000) J Biol Chem. 275 (11):7439-42.; Horowitz DS., et al. (2002) EMBO. 21 (3):470-80.;

NCBI and Uniprot Product Information

• NCBI GI #: 5454154
• NCBI GeneID: 10465
• NCBI Accession #: NP_006338
• NCBI GenBank Nucleotide #: NM_006347.3
• UniProt Accession #: O43447; A6NNE7
• Molecular Weight: 19.2 kDa (177 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase H isoform 1
• NCBI Official Synonym Full Names: peptidylprolyl isomerase H
• NCBI Official Symbol: PPIH
• NCBI Official Synonym Symbols: CYPH; CYP-20; USA-CYP; SnuCyp-20
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase H
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase H
• UniProt Gene Name: PPIH
• UniProt Synonym Gene Names: CYP20; CYPH; PPIase H; CypH; USA-CYP

NCBI Description

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a specific component of the complex that includes pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. This protein has been shown to possess PPIase activity and may act as a protein chaperone that mediates the interactions between different proteins inside the spliceosome. [provided by RefSeq, Jul 2008]

Uniprot Description

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. May act as a chaperone.