Mouse Tyrosine Hydroxylase ELISA Kit | TH elisa kit

Mouse Tyrosine Hydroxylase (TH) ELISA Kit

Cat. Num. AAA2031974

• Gene Names: TH; TYH; DYT14; DYT5b
• Reactivity: Mouse
• Synonyms: Tyrosine Hydroxylase; Mouse Tyrosine Hydroxylase (TH) ELISA Kit; TYH; DYT5b; Tyrosine 3-Monooxygenase; Dystonia 14; TH elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of TH. No significant cross-reactivity or interference between TH and analogues was observed.
• Sequence Length: 106

• Samples: Mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Competitive
• Detection Range: 9.88-800pg/mL. The standard curve concentrations used for the ELISA's were 800pg/mL, 266.67pg/mL, 88.89pg/mL, 29.63pg/mL, 9.88pg/mL,
• Sensitivity: 3.76pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C.

Typical Testing Data/Standard Curve

Related Product Information for TH elisa kit

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of TH in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay||This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to TH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled TH and unlabeled TH (Standards or samples) with the pre-coated antibody specific to TH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of TH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of TH in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 986909
• NCBI GeneID: 7054
• Molecular Weight: 44,898 Da
• NCBI Official Full Name: tyrosine hydroxylase, partial
• NCBI Official Synonym Full Names: tyrosine hydroxylase
• NCBI Official Symbol: TH
• NCBI Official Synonym Symbols: TYH; DYT14; DYT5b
• NCBI Protein Information: tyrosine 3-monooxygenase
• UniProt Protein Name: Tyrosine 3-monooxygenase
• UniProt Gene Name: TH
• UniProt Synonym Gene Names: TYH; TH
• UniProt Entry Name: TY3H_HUMAN

NCBI Description

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: EC 1.14.16.2; Mitochondrial; Vesicle; Amino Acid Metabolism - tyrosine; Endoplasmic reticulum; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytoplasm; cytoplasmic vesicle; cytosol; dendrite; internal side of plasma membrane; melanosome membrane; mitochondrion; neuron projection; nucleus; perikaryon; smooth endoplasmic reticulum; synaptic vesicle; terminal button

Molecular Function: amino acid binding; dopamine binding; enzyme binding; ferric iron binding; ferrous iron binding; oxygen binding; protein binding; protein domain specific binding; tyrosine 3-monooxygenase activity

Biological Process: anatomical structure morphogenesis; catecholamine biosynthetic process; cerebral cortex development; circadian sleep/wake cycle; dopamine biosynthetic process; dopamine biosynthetic process from tyrosine; eating behavior; embryonic camera-type eye morphogenesis; epinephrine biosynthetic process; eye photoreceptor cell development; fatty acid metabolic process; glycoside metabolic process; heart development; heart morphogenesis; isoquinoline alkaloid metabolic process; learning; locomotory behavior; mating behavior; memory; multicellular organismal aging; neurotransmitter biosynthetic process; norepinephrine biosynthetic process; organ morphogenesis; phthalate metabolic process; phytoalexin metabolic process; pigmentation; regulation of heart contraction; response to activity; response to amphetamine; response to corticosterone stimulus; response to electrical stimulus; response to estradiol stimulus; response to ethanol; response to ether; response to herbicide; response to hypoxia; response to light stimulus; response to lipopolysaccharide; response to nutrient levels; response to peptide hormone stimulus; response to pyrethroid; response to salt stress; response to water deprivation; response to zinc ion; sensory perception of sound; social behavior; sphingolipid metabolic process; synaptic transmission, dopaminergic; synaptic vesicle amine transport; terpene metabolic process; visual perception

Disease: Segawa Syndrome, Autosomal Recessive