HAUS Augmin Like Complex Subunit 7 Recombinant Protein | HAUS7 recombinant protein

Recombinant HAUS Augmin Like Complex Subunit 7 (HAUS7)

Cat. Num. AAA2031742

• Gene Names: HAUS7; UIP1; UCHL5IP
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: HAUS Augmin Like Complex Subunit 7; Recombinant HAUS Augmin Like Complex Subunit 7 (HAUS7); HAUS7 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; QKQL QFMDKLLDMM QSLATGCSSS SSLKEHLEGA TEKNDALLGE LFSSPNLWAI LKPESDPWPL DMQSSLNKQC DDLPKAGPSA QSEGEKVADL ARQLQESATK LQTLRDQCFA QHKAGTDTST VDQKLRLVIS DFYQLILAFL QVYDDELGEC CQRPVPSLHP SGPIIQAVYQ TLASCGQLLK AVMEIADTSA EAMEAARKQE GEPNCWSSNN SGISLAARID EVTQKYKILTDRFHRGTR
• Sequence Length: 368

• Applicable Applications for HAUS7 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 30.4kDa
• Accurate Molecular Mass (KD): 27kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, T7 tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Gln12~Ile65
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 66346717
• NCBI GeneID: 55559
• NCBI Accession #: NP_059988.3
• NCBI GenBank Nucleotide #: NM_017518.7
• Molecular Weight: 37,524 Da
• NCBI Official Full Name: HAUS augmin-like complex subunit 7
• NCBI Official Synonym Full Names: HAUS augmin-like complex, subunit 7
• NCBI Official Symbol: HAUS7
• NCBI Official Synonym Symbols: UIP1; UCHL5IP
• NCBI Protein Information: HAUS augmin-like complex subunit 7
• UniProt Protein Name: HAUS augmin-like complex subunit 7
• Protein Family: HAUS augmin-like complex
• UniProt Gene Name: HAUS7
• UniProt Synonym Gene Names: UCHL5IP; UIP1
• UniProt Entry Name: HAUS7_HUMAN

NCBI Description

This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for the completion of cytokinesis. The encoded protein was identified by interaction with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]

Uniprot Description

HAUS7: Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Belongs to the HAUS7 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Deoxyribonuclease; DNA-binding; EC 3.1.11.2

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: centrosome; microtubule; cytoplasm; nucleolus; plasma membrane; spindle

Molecular Function: thioesterase binding

Biological Process: mitosis; cell division; centrosome organization and biogenesis; spindle assembly