Prolylcarboxypeptidase Recombinant Protein | PRCP recombinant protein

Recombinant Prolylcarboxypeptidase (PRCP)

Cat. Num. AAA2031422

• Gene Names: PRCP; PCP; HUMPCP
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Prolylcarboxypeptidase; Recombinant Prolylcarboxypeptidase (PRCP); PRCP recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; QP VIAIGGSYGG MLAAWFRMKY PHIVVGALAA SAPIWQLDGM VPCGEFMKIV TNDFRKSGPY CSESIRKSWN VIDKLSGSGS GLQSLTNILH LCSPLTSEKI PTLKGWIAET WVNLAMVNYP YACNFLQPLP AWPIKEVCQY LKNPNVSDTV LLQNIFQALS VYYNYSGQAA CLNISQTTTS SLGSMGWSFQ ACTEMVMPFC TNGIDDMFEP FLWDLEKYSNDCFNQWGVKP RPHWMTTMYG GKNISSHSNI IFSNGELDPW SGGGVTRDIT DTL
• Sequence Length: 496

• Applicable Applications for PRCP recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 60.5KDa
• Accurate Molecular Mass (KD): 61kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and GST-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Gln169~Leu443
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 431321
• NCBI GeneID: 5547
• Molecular Weight: 58,100 Da
• NCBI Official Full Name: prolylcarboxypeptidase
• NCBI Official Synonym Full Names: prolylcarboxypeptidase (angiotensinase C)
• NCBI Official Symbol: PRCP
• NCBI Official Synonym Symbols: PCP; HUMPCP
• NCBI Protein Information: lysosomal Pro-X carboxypeptidase
• UniProt Protein Name: Lysosomal Pro-X carboxypeptidase
• Protein Family: Lysosomal Pro-X carboxypeptidase
• UniProt Gene Name: PRCP
• UniProt Synonym Gene Names: PCP; PRCP
• UniProt Entry Name: PCP_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal prolylcarboxypeptidase, which cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

PRCP: Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH. Belongs to the peptidase S28 family.

Protein type: Protease; EC 3.4.16.2

Chromosomal Location of Human Ortholog: 11q14

Cellular Component: lysosome; plasma membrane

Molecular Function: serine carboxypeptidase activity; protein binding

Biological Process: negative regulation of systemic arterial blood pressure; plasma kallikrein-kinin cascade; proteolysis; regulation of blood vessel endothelial cell migration; angiogenesis involved in wound healing; blood coagulation; glucose homeostasis; blood coagulation, intrinsic pathway