Fucosidase Alpha L1 Recombinant Protein | FUCa1 recombinant protein

Recombinant Fucosidase Alpha L1, Tissue (FUCa1)

Cat. Num. AAA2030701

• Gene Names: FUCA1; FUCA
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Fucosidase Alpha L1; Recombinant Fucosidase Alpha L1; Tissue (FUCa1); FUCa1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 6.4 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- FDEAKFGV FIHWGVFSVP AWGSEWFWWH WQGEGRPQYQ RFMRDNYPPG FSYADFGPQF TARFFHPEEW ADLFQAAGAK YVVLTTKHHE GFTNWPSPVS WNWNSKDVGP HRDLVGELGT ALRKRNIRYG LYHSLLEWFH PLYLLDKKNG FKTQHFVSAK TMPELYDLVN SYKPDLIWSD GEWECPDTYWNSTNFLSWLY NDSPVKDEVV VNDRWGQNCS CHHGGYYNCE DKFKPQSLP
• Sequence Length: 461

• Applicable Applications for FUCa1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 31.9kDa
• Accurate Molecular Mass (KD): 32kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Phe53~Pro289
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 823671486
• NCBI GeneID: 2517
• Molecular Weight: 53,689 Da
• NCBI Official Full Name: FUCA1, partial
• NCBI Official Synonym Full Names: fucosidase, alpha-L- 1, tissue
• NCBI Official Symbol: FUCA1
• NCBI Official Synonym Symbols: FUCA
• NCBI Protein Information: tissue alpha-L-fucosidase
• UniProt Protein Name: Tissue alpha-L-fucosidase
• Protein Family: Tissue alpha-L-fucosidase
• UniProt Gene Name: FUCA1
• UniProt Synonym Gene Names: Alpha-L-fucosidase 1
• UniProt Entry Name: FUCO_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids. Mutations in this gene are associated with fucosidosis (FUCA1D), which is an autosomal recessive lysosomal storage disease. A pseudogene of this locus is present on chr 2.[provided by RefSeq, Oct 2009]

Uniprot Description

FUCA1: Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N- acetylglucosamine of the carbohydrate moieties of glycoproteins. Defects in FUCA1 are the cause of fucosidosis (FUCA1D). FUCA1D is an autosomal recessive lysosomal storage disease characterized by accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Clinical signs include facial dysmorphism, dysostosis multiplex, moderate hepatomegaly, severe intellectual deficit, deafness, and according to age, angiokeratomas. Belongs to the glycosyl hydrolase 29 family.

Protein type: Glycan Metabolism - other glycan degradation; EC 3.2.1.51; Hydrolase

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: lysosome; cytoplasm

Molecular Function: alpha-L-fucosidase activity; fucose binding

Biological Process: fucose metabolic process; glycosaminoglycan catabolic process; glycoside catabolic process

Disease: Fucosidosis