Glutaminase Recombinant Protein | GLS recombinant protein

Recombinant Glutaminase (GLS)

Cat. Num. AAA2030612

• Gene Names: GLS2; GA; GLS; LGA; hLGA
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Glutaminase; Recombinant Glutaminase (GLS); GLS recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; IPDFMSFTSH IDELYESAKK QSGGKVADYIPQLAKFSPDL WGVSVCTVDG QRHSTGDTKV PFCLQSCVKP LKYAIAVNDLGTEYVHRYVG KEPSGLRFNK LFLNEDDKPH NPMVNAGAIV VTSLIKQGVNNAEKFDYVMQ FLNKMAGNEY VGFSNATFQS ERESGDRNFA IGYYLKEKKCFPEGTDMVGI LDFYFQLCSI EVTCESASVM AATLANGGFC PITGERVLSPEAVRNTLSLM HSCGMYDFSG QFAFHVGLPA KSGVAGGILL VVPNVMGMMCWSPPLDKMGN SVKGIHFCHD LVSLCNFHNY DNL
• Sequence Length: 263

• Applicable Applications for GLS recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 38.1kDa
• Accurate Molecular Mass (KD): 38kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Ile221~Leu533
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6650608
• NCBI GeneID: 27165
• Molecular Weight: 23,055 Da
• NCBI Official Full Name: glutaminase
• NCBI Official Synonym Full Names: glutaminase 2 (liver, mitochondrial)
• NCBI Official Symbol: GLS2
• NCBI Official Synonym Symbols: GA; GLS; LGA; hLGA
• NCBI Protein Information: glutaminase liver isoform, mitochondrial
• UniProt Protein Name: Glutaminase liver isoform, mitochondrial
• Protein Family: Glutaminase
• UniProt Gene Name: GLS2
• UniProt Synonym Gene Names: GA; GLS
• UniProt Entry Name: GLSL_HUMAN

NCBI Description

The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

GLS2: Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation. Belongs to the glutaminase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Hydrolase; Amino Acid Metabolism - arginine and proline; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; Mitochondrial; Energy Metabolism - nitrogen; EC 3.5.1.2

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: protein binding; glutaminase activity

Biological Process: regulation of apoptosis; amino acid metabolic process; synaptic transmission; glutamate secretion; neurotransmitter secretion; glutamine metabolic process; amino acid biosynthetic process