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SDS-Page

GroES recombinant protein

GroES, 1-97aa, E Coli, E Coli

Gene Names
groS; ECK4136; groES; JW4102; mopB; TabB
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
GroES; 1-97aa; E Coli; CPN10; 10 kDa chaperonin; groS; mopB; 10 kDa heat shock protein mitochondrial; Chaperonin 10; Cpn 10; Early pregnancy factor; EPF; Heat shock 10kD protein 1 chaperonin 10; Heat shock 10kDa protein 1; Heat shock 10kDa protein 1 chaperonin 10; HSP 10; HSP10; HSPE 1; HSPE1; ; GroES recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 25 mM Tris-HCl (pH 7.5) 100mM NaCl, 5mM DTT, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MNIRPLHDRV IVKRKEVETK SAGGIVLTGS AAAKSTRGEV LAVGNGRILE NGEVKPLDVK VGDIVIFNDG YGVKSEKIDN EEVLIMSESD ILAIVEA
Sequence Length
97
Applicable Applications for GroES recombinant protein
SDS-PAGE
Antigen Species
E Coli
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for GroES recombinant protein
GroES protein is the co-chaperonin of GroES in E Coli and assists protein folding. GroEL mediated folding requires the co-chaperonin GroES which is essential for viability. GroES is composed of a single heptameric ring of 10kDa subunits that binds to the ends of the GroEL cylinder. GroES gene was amplified by PCR from E Coli and cloned into an expression vector. This protein was overexpressed in E Coli and was purified by using conventional chromatography techniques.
Product Categories/Family for GroES recombinant protein
References
Jue D. Wang, et al (1998) Proc. Natl. Acad. Sci. uSA 95, 12163-12168; Landry,S.J., et al (1993) Nature 364, 255-258; Xu, Z., et al (1997) Nature 388,741-750;

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10.4kDa (97aa), confirmed by MALDI-TOF.
NCBI Official Full Name
Cpn10 chaperonin GroES, small subunit of GroESL
NCBI Official Symbol
groS
NCBI Official Synonym Symbols
ECK4136; groES; JW4102; mopB; TabB
NCBI Protein Information
Cpn10 chaperonin GroES, small subunit of GroESL
UniProt Protein Name
10 kDa chaperonin
UniProt Gene Name
groS
UniProt Synonym Gene Names
groES; mopB

NCBI Description

GroS binds and regulates rpoH. [More information is available at EcoGene: EG10600]. The Escherichia coli chaperone protein GroEL (Hsp60) and its regulator GroES are necessary for the proper folding of certain proteins , . [More information is available at EcoCyc: EG10600].

Uniprot Description

Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.

Research Articles on GroES

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Product Notes

The GroES gros (Catalog #AAA203024) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GroES can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the GroES gros for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MNIRPLHDRV IVKRKEVETK SAGGIVLTGS AAAKSTRGEV LAVGNGRILE NGEVKPLDVK VGDIVIFNDG YGVKSEKIDN EEVLIMSESD ILAIVEA. It is sometimes possible for the material contained within the vial of "GroES, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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