Semaphorin 4D Recombinant Protein | SEMA4D recombinant protein

Recombinant Semaphorin 4D (SEMA4D)

Cat. Num. AAA2029587

• Gene Names: SEMA4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Semaphorin 4D; Recombinant Semaphorin 4D (SEMA4D); SEMA4D recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; LGKSED GKGRCPFDPA HSYTSVMVGG ELYSGTSYNF LGSEPIISRN SSHSPLRTEY AIPWLNEPSF VFADVIHKSP DGTEAEDDKV YFFFTEVSVE YEFVFKLMIP RVARVCKGDQ GGLRTLQKKW TSFLKARLIC SRPDSGLVFN ILQDVFVLRA PGLKEPVFYA VFTPQLNNVG LSAVCAYTLS TVEAVFSRGK YMQSATVEQS HTKWVRYNGP VPTPRPGACI DSEARAANYT SSLNLPDKTL QFVKDHPLMD DSVTPIDNRP KLIKKDVNYT QIVVDRTQAL DGTFYDVMFLSTDRGALHKA VILAKEVHVV EETQLFQDFE PVLSLLLSSK KGRKFVYAGS NSGV
• Sequence Length: 862

• Applicable Applications for SEMA4D recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 68.9kDa
• Accurate Molecular Mass (KD): 69kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, GST-tag, S-tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Leu145~Gly494
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 214010218
• NCBI GeneID: 10507
• NCBI Accession #: NP_006369.3
• NCBI GenBank Nucleotide #: NM_006378.3
• Molecular Weight: 82,164 Da
• NCBI Official Full Name: semaphorin-4D isoform 1
• NCBI Official Synonym Full Names: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• NCBI Official Symbol: SEMA4D
• NCBI Official Synonym Symbols: CD100; SEMAJ; coll-4; C9orf164; M-sema-G
• NCBI Protein Information: semaphorin-4D
• UniProt Protein Name: Semaphorin-4D
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA4D
• UniProt Synonym Gene Names: C9orf164; CD100; SEMAJ
• UniProt Entry Name: SEM4D_HUMAN

Uniprot Description

SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q22.2

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor activity; semaphorin receptor binding; receptor binding

Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; axon guidance; regulation of dendrite morphogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell adhesion; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell shape; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of osteoblast differentiation; immune response; positive regulation of protein amino acid phosphorylation; cell adhesion; positive regulation of collateral sprouting; negative regulation of apoptosis; positive regulation of cell migration; regulation of cell projection organization and biogenesis