SDS-Page
Glutamate Cysteine Ligase Recombinant Protein | GCLM recombinant protein
Recombinant Glutamate Cysteine Ligase, Modifier Subunit (GCLM)
Gene Names
GSH1; ATECS1; CAD2; CADMIUM SENSITIVE 2; F7H19.290; F7H19_290; GAMMA-GLUTAMYL-CYSTEINE SYNTHETASE; GAMMA-GLUTAMYLCYSTEINE SYNTHETASE; glutamate-cysteine ligase; GLUTAMATE-CYSTEINE LIGASE; GSH1; GSHA; PAD2; PHYTOALEXIN DEFICIENT 2; RML1; ROOT MERISTEMLESS 1
Applications
SDS-Page, Western Blot
Purity
>95%
Synonyms
Glutamate Cysteine Ligase; Recombinant Glutamate Cysteine Ligase; Modifier Subunit (GCLM); GCLM recombinant protein
Host
E.coli
Purity/Purification
>95%
Concentration
Original concentration: 200 ug/mL (varies by lot)
Applicable Applications for GCLM recombinant protein
Positive Control, Immunogen, SDS-PAGE,WB
(May be suitable for use in other assays to be determined by the end user.)
(May be suitable for use in other assays to be determined by the end user.)
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Ser40~Ser251
Tags
N-terminal His Tag
Traits
Freeze-dried powder
Buffer
100mMNaHCO3, 500mMNaCl, pH8.3, containing 0.01% SKL, 5% Trehalose.
Predicted isoelectric point
4.9
Predicted Molecular Mass
27.6kDa
Accurate Molecular Mass
28kDa as determined by SDS-PAGE reducing conditions.
Usage
Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
glutamate-cysteine ligase
NCBI Official Symbol
GSH1
NCBI Official Synonym Symbols
ATECS1; CAD2; CADMIUM SENSITIVE 2; F7H19.290; F7H19_290; GAMMA-GLUTAMYL-CYSTEINE SYNTHETASE; GAMMA-GLUTAMYLCYSTEINE SYNTHETASE; glutamate-cysteine ligase; GLUTAMATE-CYSTEINE LIGASE; GSH1; GSHA; PAD2; PHYTOALEXIN DEFICIENT 2; RML1; ROOT MERISTEMLESS 1
NCBI Protein Information
glutamate-cysteine ligase
UniProt Protein Name
Glutamate--cysteine ligase, chloroplastic
Protein Family
UniProt Gene Name
GSH1
UniProt Synonym Gene Names
CAD2; GCL; PAD2; RML1; GCS; AtGCL
UniProt Entry Name
GSH1_ARATH
NCBI Description
Encodes the enzyme glutamate-cysteine ligase catalyzing the first, and rate-limiting, step of glutathione biosynthesis. Required for cell proliferation at the root tip. Involved in susceptibility to the bacterial pathogen Pseudomonas syringae. Mutants are phytoalexin defective.
Uniprot Description
Seems to play an important role in controlling the expression of resistance responses like the regulation of salicylic acid (SA) and phytoalexin (camalexin) production. Involved in resistance to fungal and bacterial pathogens. Required for the regulation of cell proliferation in root apical meristems through the GSH-dependent developmental pathway. Also participates in the detoxification process, the antioxidant response and is essential for embryo development and proper seed maturation.
Research Articles on GCLM
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Product Notes
The GCLM gsh1 (Catalog #AAA2029337) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Glutamate Cysteine Ligase can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE,WB (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the GCLM gsh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutamate Cysteine Ligase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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