Western Blot (WB)
(Western Blot: Sample: Human A431 cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Human GCLM Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human Glutamate Cysteine Ligase Polyclonal Antibody | anti-GCLM antibody
Polyclonal Antibody to Glutamate Cysteine Ligase, Modifier Subunit (GCLM)
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Human A431 cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Human GCLM Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human A431 cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Human GCLM Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant GCLM, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant GCLM, Human.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC)
(FITC staining on IHC-P Simple: Mcf7 cells))
Immunohistochemistry (IHC)
(FITC staining on IHC-P Simple: Mcf7 cells))
NCBI and Uniprot Product Information
NCBI Description
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
Uniprot Description
GCLM: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq, Jul 2008]
Protein type: Other Amino Acids Metabolism - glutathione
Chromosomal Location of Human Ortholog: 1p22.1
Cellular Component: cytosol; glutamate-cysteine ligase complex
Molecular Function: enzyme regulator activity; glutamate-cysteine ligase activity; glutamate-cysteine ligase catalytic subunit binding; protein heterodimerization activity
Biological Process: aging; apoptotic mitochondrial changes; cysteine metabolic process; glutamate metabolic process; glutathione biosynthetic process; negative regulation of neuron apoptosis; positive regulation of glutamate-cysteine ligase activity; regulation of blood vessel size; regulation of mitochondrial depolarization; response to activity; response to drug; response to nitrosative stress; response to nutrient; response to oxidative stress
Disease: Myocardial Infarction, Susceptibility To
Research Articles on GCLM
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Product Notes
The GCLM gclm (Catalog #AAA2027356) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glutamate Cysteine Ligase, Modifier Subunit (GCLM) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glutamate Cysteine Ligase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the GCLM gclm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutamate Cysteine Ligase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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