Nestin Recombinant Protein | NES recombinant protein

Recombinant Nestin (NES)

Cat. Num. AAA2029165

• Gene Names: NES; Nbla00170
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Nestin; Recombinant Nestin (NES); NES recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 10.3 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LAR RLGEAWRGAV RGYQERVAHM ETSLGQARER LGRAVQGARE GRLELQQLQA ERGGLLERRA ALEQRLEGRW QERLRATEKF QLAVEALEQE KQGLQSQIAQ VLEGRQQLAH LKMSLSLEVA TYRTLLEAEN SRLQTPGGGS KTSLSFQDPK LELQFPRTPE GRRLGSLLPV LSPTSLPSPLPATLETPVPA FLKNQEFLQA RTPTLASTPI PPTPQAPSP
• Sequence Length: 1621

• Applicable Applications for NES recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 28.5kDa
• Accurate Molecular Mass (KD): 31kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Leu178~Pro399
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 38176300
• NCBI GeneID: 10763
• NCBI Accession #: NP_006608.1
• NCBI GenBank Nucleotide #: NM_006617.1
• Molecular Weight: 177,439 Da
• NCBI Official Full Name: nestin
• NCBI Official Synonym Full Names: nestin
• NCBI Official Symbol: NES
• NCBI Official Synonym Symbols: Nbla00170
• NCBI Protein Information: nestin
• UniProt Protein Name: Nestin
• Protein Family: Nestin
• UniProt Gene Name: NES
• UniProt Entry Name: NEST_HUMAN

NCBI Description

This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]

Uniprot Description

nestin: an intermediate filament protein. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: intermediate filament cytoskeleton; cytoplasm; intermediate filament

Molecular Function: intermediate filament binding; structural molecule activity

Biological Process: positive regulation of intermediate filament depolymerization; central nervous system development; negative regulation of catalytic activity; cell projection morphogenesis; negative regulation of neuron apoptosis; brain development; G2/M transition of mitotic cell cycle; negative regulation of protein binding; embryonic camera-type eye development