Rabbit anti-Mouse Matrix Metalloproteinase 20 Polyclonal Antibody | anti-MMP20 antibody

Polyclonal Antibody to Matrix Metalloproteinase 20 (MMP20)

Cat. Num. AAA2028270

• Reactivity: Mouse
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: Matrix Metalloproteinase 20; Polyclonal Antibody; Polyclonal Antibody to Matrix Metalloproteinase 20 (MMP20); anti-MMP20 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against MMP20. It has beenselected for its ability to recognize MMP20 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Liquid
• Concentration: 0.54mg/ml (varies by lot)
• Sequence Length: 483

• Applicable Applications for anti-MMP20 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western blotting: 0.2-2ug/mL;1:250-2500; Immunohistochemistry: 5-20ug/mL;1:25-100; Immunocytochemistry: 5-20ug/mL;1:25-100; Optimal working dilutions must be determined by end user.
• Fragment: MMP20 (Val137~Tyr398)
• Organism Species: Mus musculus (Mouse)
• Cross Reactivity: Mouse
• Conjugate: No Conjugate
• Immunogen: Recombinant MMP20 (Val137~Tyr398) expressed in E Coli.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2059880
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 7305275
• NCBI GeneID: 30800
• NCBI Accession #: NP_038931.1
• NCBI GenBank Nucleotide #: NM_013903.2
• UniProt Accession #: P57748; A7MCV5
• Molecular Weight: 54,373 Da
• NCBI Official Full Name: matrix metalloproteinase-20 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 20 (enamelysin)
• NCBI Official Symbol: Mmp20
• NCBI Protein Information: matrix metalloproteinase-20
• UniProt Protein Name: Matrix metalloproteinase-20
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: Mmp20
• UniProt Synonym Gene Names: MMP-20

NCBI Description

This gene encodes a member of the matrix metalloproteinase family of zinc-dependent endopeptidases capable of degrading extracellular matrix proteins. This gene is expressed specifically in the ameloblasts and odontoblasts, and the encoded protein is an inactive zymogen that requires proteolytic removal of a N-terminal propeptide to become enzymatically active. Mice lacking the encoded protein display an amelogenesis imperfecta phenotype. [provided by RefSeq, Oct 2015]

Uniprot Description

MMP20: Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation. Defects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2). AI2A2 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.-; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9|9 A1

Cellular Component: extracellular matrix; extracellular region; proteinaceous extracellular matrix

Molecular Function: hydrolase activity; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding

Biological Process: extracellular matrix disassembly; protein catabolic process; proteolysis