Western Blot (WB)
(Western Blot:Sample: Lane 1: Human A549 Cells;Lane 2: Porcine Heart Tissue;Lane 3: Human Jurkat Cells;Lane 4: Porcine Skeletal Muscle Tissue.)
Rabbit Phosphofructokinase Polyclonal Antibody | anti-PFKP antibody
Polyclonal Antibody to Phosphofructokinase, Platelet (PFKP)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot:Sample: Lane 1: Human A549 Cells;Lane 2: Porcine Heart Tissue;Lane 3: Human Jurkat Cells;Lane 4: Porcine Skeletal Muscle Tissue.)
Western Blot (WB)
(Western Blot:Sample: Lane 1: Human A549 Cells;Lane 2: Porcine Heart Tissue;Lane 3: Human Jurkat Cells;Lane 4: Porcine Skeletal Muscle Tissue.)
Western Blot (WB)
(Western Blot:Sample: Recombinant PFKP, Human.)
Western Blot (WB)
(Western Blot:Sample: Recombinant PFKP, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Liver Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human PFKP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Liver Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human PFKP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Breast Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Breast Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Lung Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Lung Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Brain Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Brain Tissue)
Knockout Validation
(Knockout Validation:Lane 1: Wild-type A549 cell lysate;Lane 2: PFKP knockout A549 cell lysate;Predicted MW: 85kdObserved MW: 85kdPrimary Ab: 3ug/ml Rabbit Anti-Human PFKP AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation:Lane 1: Wild-type A549 cell lysate;Lane 2: PFKP knockout A549 cell lysate;Predicted MW: 85kdObserved MW: 85kdPrimary Ab: 3ug/ml Rabbit Anti-Human PFKP AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
Uniprot Description
PFKP: phosphofructokinase, platelet type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts.
Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.11; Kinase, other
Chromosomal Location of Human Ortholog: 10p15.2
Cellular Component: cytoplasm; cytosol; extracellular exosome; extracellular matrix; membrane; nucleus
Molecular Function: 6-phosphofructokinase activity; ATP binding; cadherin binding; metal ion binding; protein complex binding
Biological Process: fructose 6-phosphate metabolic process
Research Articles on PFKP
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Product Notes
The PFKP pfkp (Catalog #AAA2027874) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Phosphofructokinase, Platelet (PFKP) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphofructokinase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the PFKP pfkp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphofructokinase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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