Western Blot (WB)
(Western Blot: Sample: Mouse Spleen lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse NCF2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Neutrophil Cytosolic Factor 2 Polyclonal Antibody | anti-NCF2 antibody
Polyclonal Antibody to Neutrophil Cytosolic Factor 2 (NCF2)
Gene Names
Ncf2; NOXA2; Ncf-2; p67phox
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Neutrophil Cytosolic Factor 2; Polyclonal Antibody; Polyclonal Antibody to Neutrophil Cytosolic Factor 2 (NCF2); anti-NCF2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against NCF2. It has beenselected for its ability to recognize NCF2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KVHYKYT VVMETRLGLP YSQLRNMVSK KLALSPEHTK LSYRRRDSHE LLLLSEESMK DAWGQVKNYC LTLWCEHTVG DQGLIDEPIQ RENSDASKQT TEPQPKEGTQ VVAIFSYEAA QPEDLEFVEG DVILVLSHVN
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KVHYKYT VVMETRLGLP YSQLRNMVSK KLALSPEHTK LSYRRRDSHE LLLLSEESMK DAWGQVKNYC LTLWCEHTVG DQGLIDEPIQ RENSDASKQT TEPQPKEGTQ VVAIFSYEAA QPEDLEFVEG DVILVLSHVN
Sequence Length
543
Applicable Applications for anti-NCF2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
NCF2 (Lys354~Val525)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant NCF2 (Lys354~Val525) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2040733
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Spleen lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse NCF2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Spleen lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse NCF2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,485 Da
NCBI Official Full Name
neutrophil cytosol factor 2
NCBI Official Synonym Full Names
neutrophil cytosolic factor 2
NCBI Official Symbol
Ncf2
NCBI Official Synonym Symbols
NOXA2; Ncf-2; p67phox
NCBI Protein Information
neutrophil cytosol factor 2
UniProt Protein Name
Neutrophil cytosol factor 2
Protein Family
UniProt Gene Name
Ncf2
UniProt Synonym Gene Names
NCF-2
Uniprot Description
p67phox: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Interacts with SYTL1 and RAC1. Interacts with NCF4. May interact with NOXO1. Belongs to the NCF2/NOXA1 family.
Protein type: Oxidoreductase
Chromosomal Location of Human Ortholog: 1 G3|1 65.05 cM
Cellular Component: acrosomal vesicle; cytoplasm; cytosol; membrane; NADPH oxidase complex; nucleolus
Molecular Function: protein binding; protein C-terminus binding; Rac GTPase binding; superoxide-generating NADPH oxidase activator activity; superoxide-generating NADPH oxidase activity
Biological Process: cellular response to hormone stimulus; NADP catabolic process; phagocytosis; positive regulation of blood pressure; positive regulation of neuron apoptotic process; respiratory burst; response to glucose stimulus; response to lipopolysaccharide; response to organic cyclic compound; superoxide anion generation; superoxide metabolic process
Research Articles on NCF2
Similar Products
Product Notes
The NCF2 ncf2 (Catalog #AAA2027661) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Neutrophil Cytosolic Factor 2 (NCF2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Neutrophil Cytosolic Factor 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the NCF2 ncf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KVHYKYT VVMETRLGLP YSQLRNMVSK KLALSPEHTK LSYRRRDSHE LLLLSEESMK DAWGQVKNYC LTLWCEHTVG DQGLIDEPIQ RENSDASKQT TEPQPKEGTQ VVAIFSYEAA QPEDLEFVEG DVILVLSHVN. It is sometimes possible for the material contained within the vial of "Neutrophil Cytosolic Factor 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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