Rabbit anti-Human Mothers Against Decapentaplegic Homolog 5 Polyclonal Antibody | anti-Smad5 antibody

Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 5 (Smad5)

Cat. Num. AAA2026976

• Gene Names: SMAD5; DWFC; JV5-1; MADH5
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: Mothers Against Decapentaplegic Homolog 5; Polyclonal Antibody; Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 5 (Smad5); anti-Smad5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against Smad5. It has beenselected for its ability to recognize Smad5 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Liquid
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- G PGSPFQLPAD TPPPAYMPPD DQMGQDNSQP MDTSNNMIPQ IMPSISSRDV QPVAYEEPKH WCSIVYYELN NRVGEAFHAS STSVLVDGFT DPSNNKSRFC LGLLSNVNRN STIENTRRHI GKGVHLYYVG GEVYAECLSD SSIFVQSRNC NFHHGFHPTT VCKIPSSCSL KIFNNQEFAQ LLAQSVNHGF EAVYELTKMC
• Sequence Length: 465

• Applicable Applications for anti-Smad5 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western blotting: 1-5ug/ml; Immunocytochemistry in formalin fixed cells: 5-20ug/ml; Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml; Immunohistochemistry in paraffin section: 5-20ug/ml; Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml; Optimal working dilutions must be determined by end user.
• Fragment: Smad5 (Gly210~Val464)
• Organism Species: Homo sapiens (Human)
• Cross Reactivity: Human
• Conjugate: No Conjugate
• Immunogen: Recombinant Smad5 (Gly210~Val464) expressed in E Coli.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2063344
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Human K562 Cells; Lane2: Human Hela Cells; Lane3: Human Jurkat Cells.)

Western Blot (WB)

(Western Blot: Sample: Recombinant Smad5, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Rectum CancerTissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Lung Cancer Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Breast CancerTissue)

NCBI and Uniprot Product Information

• NCBI GI #: 47778929
• NCBI GeneID: 4090
• NCBI Accession #: NP_001001419.1
• NCBI GenBank Nucleotide #: NM_001001419.2
• UniProt Accession #: Q99717; O14688; Q15798; Q9UQA1
• Molecular Weight: 52,258 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 5
• NCBI Official Synonym Full Names: SMAD family member 5
• NCBI Official Symbol: SMAD5
• NCBI Official Synonym Symbols: DWFC; JV5-1; MADH5
• NCBI Protein Information: mothers against decapentaplegic homolog 5
• UniProt Protein Name: Mothers against decapentaplegic homolog 5
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: SMAD5
• UniProt Synonym Gene Names: MADH5; MAD homolog 5; Mothers against DPP homolog 5; SMAD 5; Smad5; hSmad5

NCBI Description

The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

SMAD5: transcription factor phosphorylated and activated by bone morphogenetic protein receptor kinases. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where they regulate transcription of target genes. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: cytoplasm; cytosol; integral component of membrane; intracellular; nucleoplasm; nucleus; protein complex; SMAD protein complex; transcription factor complex

Molecular Function: DEAD/H-box RNA helicase binding; DNA binding transcription factor activity; metal ion binding; protein binding; receptor signaling protein activity; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; ubiquitin protein ligase binding

Biological Process: BMP signaling pathway; cardiac muscle contraction; cartilage development; cellular response to organic cyclic compound; embryonic pattern specification; erythrocyte differentiation; germ cell development; Mullerian duct regression; negative regulation of transcription from RNA polymerase II promoter; osteoblast fate commitment; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus; positive regulation of transcription, DNA-templated; protein amino acid phosphorylation; signal transduction; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; ureteric bud development