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Western Blot (WB)
(Western Blot: Sample: Mouse Liver Tissue.)
Rabbit anti-Mouse Glucokinase Regulatory Protein Polyclonal Antibody | anti-GKRP antibody
Polyclonal Antibody to Glucokinase Regulatory Protein (GKRP)
Gene Names
Gckr; GKRP
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Glucokinase Regulatory Protein; Polyclonal Antibody; Polyclonal Antibody to Glucokinase Regulatory Protein (GKRP); anti-GKRP antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against GKRP. It has beenselected for its ability to recognize GKRP in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GEWE LSGYEAAVPI TEKSNPLTRN LDKADAEKIV QLLGQCDAEI FQEEGQIMPT YQRLYSESVL TTMLQVAGKV QEVLKEPDGG LVVLSGGGTS GRMAFLMSVS FNQLMKGLGQ KPLYTYLIAG GDRSVVASRE RTEDSALHGI EELKKVAAGK KRVVVIGISV GLSAPFVAGQ MDYCMDNTAV FLPVLVGFNP VSMARNDPIE DWRSTFRQVA ERMQKMQEKQ EAFVLNPAIG PEGLSGSSRM K
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GEWE LSGYEAAVPI TEKSNPLTRN LDKADAEKIV QLLGQCDAEI FQEEGQIMPT YQRLYSESVL TTMLQVAGKV QEVLKEPDGG LVVLSGGGTS GRMAFLMSVS FNQLMKGLGQ KPLYTYLIAG GDRSVVASRE RTEDSALHGI EELKKVAAGK KRVVVIGISV GLSAPFVAGQ MDYCMDNTAV FLPVLVGFNP VSMARNDPIE DWRSTFRQVA ERMQKMQEKQ EAFVLNPAIG PEGLSGSSRM K
Sequence Length
625
Applicable Applications for anti-GKRP antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-5ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Fragment
GKRP (Gly17~Lys261)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant GKRP (Gly17~Lys261) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2054347
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Liver Tissue.)
Western Blot (WB)
(Western Blot: Sample: Mouse Liver Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant GKRP, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant GKRP, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Intestine Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Intestine Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
64,679 Da
NCBI Official Full Name
glucokinase regulatory protein
NCBI Official Synonym Full Names
glucokinase regulatory protein
NCBI Official Symbol
Gckr
NCBI Official Synonym Symbols
GKRP
NCBI Protein Information
glucokinase regulatory protein
UniProt Protein Name
Glucokinase regulatory protein
Protein Family
UniProt Gene Name
Gckr
UniProt Synonym Gene Names
Glucokinase regulator
Uniprot Description
GCKR: a key regulator of glucokinase in the liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen. When expressed alone, both glucokinase and GCKR localize predominantly to the cytoplasm. Co-expression induces both proteins to localize in the nucleus.
Protein type: Inhibitor; Mitochondrial
Chromosomal Location of Human Ortholog: 5 B1|5 17.27 cM
Cellular Component: cytoplasm; cytosol; mitochondrion; nucleoplasm; nucleus
Molecular Function: carbohydrate binding; enzyme binding; enzyme inhibitor activity; protein domain specific binding
Biological Process: carbohydrate metabolic process; cell glucose homeostasis; negative regulation of catalytic activity; negative regulation of glucokinase activity; positive regulation of glucokinase activity; protein import into nucleus, translocation; response to fructose stimulus; urate metabolic process
Research Articles on GKRP
Similar Products
Product Notes
The GKRP gckr (Catalog #AAA2026941) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glucokinase Regulatory Protein (GKRP) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Glucokinase Regulatory Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-5ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the GKRP gckr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GEWE LSGYEAAVPI TEKSNPLTRN LDKADAEKIV QLLGQCDAEI FQEEGQIMPT YQRLYSESVL TTMLQVAGKV QEVLKEPDGG LVVLSGGGTS GRMAFLMSVS FNQLMKGLGQ KPLYTYLIAG GDRSVVASRE RTEDSALHGI EELKKVAAGK KRVVVIGISV GLSAPFVAGQ MDYCMDNTAV FLPVLVGFNP VSMARNDPIE DWRSTFRQVA ERMQKMQEKQ EAFVLNPAIG PEGLSGSSRM K. It is sometimes possible for the material contained within the vial of "Glucokinase Regulatory Protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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