Mouse Neutrophil Gelatinase Associated Lipocalin (NGAL) CLIA Kit | NGAL clia kit

Mouse Neutrophil Gelatinase Associated Lipocalin (NGAL) CLIA Kit

Cat. Num. AAA2025279

• Gene Names: LCN2; 24p3; MSFI; NGAL
• Reactivity: Mouse
• Synonyms: Neutrophil Gelatinase Associated Lipocalin (NGAL); Mouse Neutrophil Gelatinase Associated Lipocalin (NGAL) CLIA Kit; LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2; NGAL clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neutrophil Gelatinase Associated Lipocalin (NGAL).; No significant cross-reactivity or interference between Neutrophil Gelatinase Associated Lipocalin (NGAL) and analogues was observed.
• Sequence Length: 198

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Urine, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 27.4-20,000pg/mL
• Sensitivity: < 10.2pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neutrophil Gelatinase Associated Lipocalin (NGAL) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neutrophil Gelatinase Associated Lipocalin (NGAL) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NGAL clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Neutrophil Gelatinase Associated Lipocalin (NGAL). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Neutrophil Gelatinase Associated Lipocalin (NGAL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Neutrophil Gelatinase Associated Lipocalin (NGAL) level in the sample or standard.;

Product Categories/Family for NGAL clia kit

Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 38455402
• NCBI GeneID: 3934
• NCBI Accession #: NP_005555.2
• NCBI GenBank Nucleotide #: NM_005564.3
• Molecular Weight: 22,457 Da
• NCBI Official Full Name: neutrophil gelatinase-associated lipocalin
• NCBI Official Synonym Full Names: lipocalin 2
• NCBI Official Symbol: LCN2
• NCBI Official Synonym Symbols: 24p3; MSFI; NGAL
• NCBI Protein Information: neutrophil gelatinase-associated lipocalin
• UniProt Protein Name: Neutrophil gelatinase-associated lipocalin
• UniProt Gene Name: LCN2
• UniProt Synonym Gene Names: HNL; NGAL; NGAL
• UniProt Entry Name: NGAL_HUMAN

Uniprot Description

LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function: protein homodimerization activity; protease binding; transporter activity; iron ion binding

Biological Process: response to drug; cellular response to nutrient levels; cellular iron ion homeostasis; response to virus; siderophore transport; positive regulation of cell projection organization and biogenesis; innate immune response; ion transport; response to herbicide; transmembrane transport