Mouse Methionyl Aminopeptidase 2 (METAP2) ELISA Kit | METAP2 elisa kit

Mouse Methionyl Aminopeptidase 2 (METAP2) ELISA Kit

Cat. Num. AAA2025199

• Gene Names: METAP2; p67; MAP2; MNPEP; p67eIF2
• Reactivity: Mouse
• Synonyms: Methionyl Aminopeptidase 2 (METAP2); Mouse Methionyl Aminopeptidase 2 (METAP2) ELISA Kit; MNPEP; p67; p67eIF2; Peptidase M; Initiation factor 2-associated 67 kDa glycoprotein; METAP2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Methionyl Aminopeptidase 2 (METAP2).; No significant cross-reactivity or interference between Methionyl Aminopeptidase 2 (METAP2) and analogues was observed.
• Sequence Length: 478

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.063ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Methionyl Aminopeptidase 2 (METAP2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Methionyl Aminopeptidase 2 (METAP2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for METAP2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methionyl Aminopeptidase 2 (METAP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methionyl Aminopeptidase 2 (METAP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methionyl Aminopeptidase 2 (METAP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methionyl Aminopeptidase 2 (METAP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for METAP2 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 15489376
• NCBI GeneID: 10988
• Molecular Weight: 50,497 Da
• NCBI Official Full Name: Methionyl aminopeptidase 2
• NCBI Official Synonym Full Names: methionyl aminopeptidase 2
• NCBI Official Symbol: METAP2
• NCBI Official Synonym Symbols: p67; MAP2; MNPEP; p67eIF2
• NCBI Protein Information: methionine aminopeptidase 2
• UniProt Protein Name: Methionine aminopeptidase 2
• UniProt Gene Name: METAP2
• UniProt Synonym Gene Names: MAP 2; MetAP 2; p67; p67eIF2
• UniProt Entry Name: MAP2_HUMAN

NCBI Description

This gene is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2. [provided by RefSeq, Jul 2008]

Uniprot Description

METAP2: Removes the N-terminal methionine from nascent proteins. The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. Interacts strongly with the eIF-2 gamma-subunit EIF2S3. Binds EIF2S1 at low magnesium concentrations. Belongs to the peptidase M24A family.

Protein type: Protease; EC 3.4.11.18

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: metalloexopeptidase activity; metal ion binding; aminopeptidase activity

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; regulation of rhodopsin mediated signaling; peptidyl-methionine modification; protein processing; proteolysis; N-terminal protein amino acid modification