Human Vesicular Acetylcholine Transporter (VAChT) ELISA Kit | VAChT elisa kit

Human Vesicular Acetylcholine Transporter (VAChT) ELISA Kit

Cat. Num. AAA2024606

• Gene Names: SLC18A3; VACHT
• Reactivity: Human
• Synonyms: Vesicular Acetylcholine Transporter (VAChT); Human Vesicular Acetylcholine Transporter (VAChT) ELISA Kit; SLC18A3; Solute Carrier Family 18 Member 3; Vesicular Acetylcholine; VAChT elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Vesicular Acetylcholine Transporter (VAChT).; No significant cross-reactivity or interference between Vesicular Acetylcholine Transporter (VAChT) and analogues was observed.
• Sequence Length: 532

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.117ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vesicular Acetylcholine Transporter (VAChT) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vesicular Acetylcholine Transporter (VAChT) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VAChT elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vesicular Acetylcholine Transporter (VAChT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vesicular Acetylcholine Transporter (VAChT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vesicular Acetylcholine Transporter (VAChT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vesicular Acetylcholine Transporter (VAChT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for VAChT elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 118582257
• NCBI GeneID: 6572
• NCBI Accession #: NP_003046.2
• NCBI GenBank Nucleotide #: NM_003055.2
• UniProt Accession #: Q16572; B2R7S1
• Molecular Weight: 56,903 Da
• NCBI Official Full Name: vesicular acetylcholine transporter
• NCBI Official Synonym Full Names: solute carrier family 18 (vesicular acetylcholine transporter), member 3
• NCBI Official Symbol: SLC18A3
• NCBI Official Synonym Symbols: VACHT
• NCBI Protein Information: vesicular acetylcholine transporter
• UniProt Protein Name: Vesicular acetylcholine transporter
• Protein Family: Vesicular acetylcholine transporter
• UniProt Gene Name: SLC18A3
• UniProt Synonym Gene Names: VACHT; VAChT
• UniProt Entry Name: VACHT_HUMAN

NCBI Description

This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SLC18A3: Involved in acetylcholine transport into synaptic vesicles. Belongs to the major facilitator superfamily. Vesicular transporter family.

Protein type: Transporter, SLC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: acetylcholine transmembrane transporter activity

Biological Process: synaptic transmission; acetylcholine transport; neurotransmitter secretion