Human NFKB Repressing Factor (NKRF) ELISA Kit | NKRF elisa kit

Human NFKB Repressing Factor (NKRF) ELISA Kit

Cat. Num. AAA2024570

• Gene Names: NKRF; NRF; ITBA4
• Reactivity: Human
• Synonyms: NFKB Repressing Factor (NKRF); Human NFKB Repressing Factor (NKRF) ELISA Kit; ITBA4; NRF; NF-Kappa B Repressing Factor; Transcription factor NRF; NKRF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NFKB Repressing Factor (NKRF).; No significant cross-reactivity or interference between NFKB Repressing Factor (NKRF) and analogues was observed.
• Sequence Length: 705

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.126ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NFKB Repressing Factor (NKRF) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NFKB Repressing Factor (NKRF) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NKRF elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NFKB Repressing Factor (NKRF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NFKB Repressing Factor (NKRF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NFKB Repressing Factor (NKRF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NFKB Repressing Factor (NKRF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NKRF elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 291084505
• NCBI GeneID: 55922
• NCBI Accession #: NP_001166958.1
• NCBI GenBank Nucleotide #: NM_001173487.1
• UniProt Accession #: O15226; Q4VC41; Q9UJ91; G3V1N1
• Molecular Weight: 79,496 Da
• NCBI Official Full Name: NF-kappa-B-repressing factor isoform 1
• NCBI Official Synonym Full Names: NFKB repressing factor
• NCBI Official Symbol: NKRF
• NCBI Official Synonym Symbols: NRF; ITBA4
• NCBI Protein Information: NF-kappa-B-repressing factor
• UniProt Protein Name: NF-kappa-B-repressing factor
• Protein Family: NF-kappa-B-repressing factor
• UniProt Gene Name: NKRF
• UniProt Synonym Gene Names: ITBA4; NRF; NFkB-repressing factor
• UniProt Entry Name: NKRF_HUMAN

NCBI Description

This gene encodes a transcriptional repressor that interacts with specific negative regulatory elements to mediate transcriptional repression of certain nuclear factor kappa B responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

NKRF: Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription.

Protein type: Transcription, coactivator/corepressor; RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: Xq24

Cellular Component: endoplasmic reticulum; nucleolus; nucleus

Molecular Function: protein binding; DNA binding

Biological Process: transcription, DNA-dependent; negative regulation of transcription, DNA-dependent