Human ISL LIM Homeobox Protein 1 (ISL1) ELISA Kit | ISL1 elisa kit

Human ISL LIM Homeobox Protein 1 (ISL1) ELISA Kit

Cat. Num. AAA2024482

• Gene Names: ISL1; Isl-1; ISLET1
• Reactivity: Human
• Synonyms: ISL LIM Homeobox Protein 1 (ISL1); Human ISL LIM Homeobox Protein 1 (ISL1) ELISA Kit; Islet-1; ISL1 Transcription Factor; LIM/Homeodomain; Insulin gene enhancer protein ISL-1; ISL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ISL LIM Homeobox Protein 1 (ISL1).; No significant cross-reactivity or interference between ISL LIM Homeobox Protein 1 (ISL1) and analogues was observed.
• Sequence Length: 349

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.063ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ISL LIM Homeobox Protein 1 (ISL1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ISL LIM Homeobox Protein 1 (ISL1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ISL1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ISL LIM Homeobox Protein 1 (ISL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ISL LIM Homeobox Protein 1 (ISL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ISL LIM Homeobox Protein 1 (ISL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ISL LIM Homeobox Protein 1 (ISL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ISL1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 22658390
• NCBI GeneID: 3670
• UniProt Accession #: P61371; P20663; P47894
• Molecular Weight: 39,036 Da
• NCBI Official Full Name: ISL LIM homeobox 1
• NCBI Official Synonym Full Names: ISL LIM homeobox 1
• NCBI Official Symbol: ISL1
• NCBI Official Synonym Symbols: Isl-1; ISLET1
• NCBI Protein Information: insulin gene enhancer protein ISL-1
• UniProt Protein Name: Insulin gene enhancer protein ISL-1
• Protein Family: Insulin gene enhancer protein
• UniProt Gene Name: ISL1
• UniProt Synonym Gene Names: Islet-1
• UniProt Entry Name: ISL1_HUMAN

NCBI Description

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq, Jul 2008]

Uniprot Description

ISL1: Binds to one of the cis-acting domain of the insulin gene enhancer.

Protein type: Transcription factor; Nuclear receptor co-regulator; DNA-binding; Cell development/differentiation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q11.1

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus

Molecular Function: ligand-dependent nuclear receptor binding; zinc ion binding; bHLH transcription factor binding; estrogen receptor binding; chromatin binding

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of interleukin-12 production; positive regulation of interleukin-1 alpha production; negative regulation of transcription from RNA polymerase II promoter; negative regulation of estrogen receptor signaling pathway; positive regulation of tyrosine phosphorylation of Stat3 protein; pancreas development; positive regulation of cell proliferation; ventricular cardiac muscle morphogenesis; neuron fate specification; sensory system development; negative regulation of neuron apoptosis; neural crest cell migration; positive regulation of DNA binding; trigeminal nerve development; pharyngeal system development; axon regeneration; spinal cord motor neuron cell fate specification; positive regulation of interleukin-6 production; positive regulation of insulin secretion; spinal cord motor neuron differentiation; positive regulation of tumor necrosis factor production; positive regulation of interleukin-1 beta production; positive regulation of angiogenesis; negative regulation of neuron differentiation; positive regulation of interferon-gamma production; visceral motor neuron differentiation; mesenchymal cell differentiation; pituitary gland development; negative regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; peripheral nervous system neuron axonogenesis; retinal ganglion cell axon guidance