Mouse ADP Ribosyltransferase 1 (ART1) ELISA Kit | ART1 elisa kit

Mouse ADP Ribosyltransferase 1 (ART1) ELISA Kit

Cat. Num. AAA2024082

• Gene Names: ART1; RT6; ART2; ARTC1; CD296
• Reactivity: Mouse
• Synonyms: ADP Ribosyltransferase 1 (ART1); Mouse ADP Ribosyltransferase 1 (ART1) ELISA Kit; CD296; ART2; ARTC1; Mono(ADP-ribosyl)transferase 1; ADP-ribosyltransferase C2 and C3 toxin-like 1; GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1; ART1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of ADP Ribosyltransferase 1 (ART1).; No significant cross-reactivity or interference between ADP Ribosyltransferase 1 (ART1) and analogues was observed.
• Sequence Length: 327

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: < 0.32ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ADP Ribosyltransferase 1 (ART1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ADP Ribosyltransferase 1 (ART1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ART1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADP Ribosyltransferase 1 (ART1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ADP Ribosyltransferase 1 (ART1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADP Ribosyltransferase 1 (ART1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ADP Ribosyltransferase 1 (ART1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ART1 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 84627545
• NCBI GeneID: 417
• UniProt Accession #: P52961; Q6NTD2; Q96KT9
• Molecular Weight: 36,335 Da
• NCBI Official Full Name: ADP-ribosyltransferase 1
• NCBI Official Synonym Full Names: ADP-ribosyltransferase 1
• NCBI Official Symbol: ART1
• NCBI Official Synonym Symbols: RT6; ART2; ARTC1; CD296
• NCBI Protein Information: GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1
• UniProt Protein Name: GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1
• Protein Family: Arrestin family protein
• UniProt Gene Name: ART1
• UniProt Synonym Gene Names: ARTC1
• UniProt Entry Name: NAR1_HUMAN

NCBI Description

ADP-ribosyltransferase catalyzes the ADP-ribosylation of arginine residues in proteins. Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. The amino acid sequence consists of predominantly hydrophobic N- and C-terminal regions, which is characteristic of glycosylphosphatidylinositol (GPI)-anchored proteins. This gene was previously designated ART2. [provided by RefSeq, Jul 2008]

Uniprot Description

ART1: Has ADP-ribosyltransferase activity toward GLP1R. Belongs to the Arg-specific ADP-ribosyltransferase family.

Protein type: Membrane protein, GPI anchor; EC 2.4.2.31; Transferase

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: sarcoplasmic reticulum membrane; cell surface; integral to plasma membrane; plasma membrane

Molecular Function: NAD(P)+-protein-arginine ADP-ribosyltransferase activity; NAD+ ADP-ribosyltransferase activity

Biological Process: protein amino acid ADP-ribosylation; innate immune response