Human Mucin 20 (MUC20) ELISA Kit | MUC20 elisa kit

Human Mucin 20 (MUC20) ELISA Kit

Cat. Num. AAA2023703

• Gene Names: MUC20; MUC-20
• Reactivity: Human
• Synonyms: Mucin 20 (MUC20); Human Mucin 20 (MUC20) ELISA Kit; MUC20 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mucin 20 (MUC20).; No significant cross-reactivity or interference between Mucin 20 (MUC20) and analogues was observed.
• Sequence Length: 674

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.063ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 20 (MUC20) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 20 (MUC20) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MUC20 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 20 (MUC20). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 20 (MUC20). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 20 (MUC20), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucin 20 (MUC20) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MUC20 elisa kit

Tumor immunity; Infection immunity; Pulmonology

NCBI and Uniprot Product Information

• NCBI GI #: 541444092
• NCBI GeneID: 200958
• NCBI Accession #: NP_001269435.1
• NCBI GenBank Nucleotide #: NM_001282506.1
• UniProt Accession #: Q8N307; Q6UX97; Q76I83; Q76I85; Q86ST8; Q8NBY6; Q96KA1; Q9P2I8
• Molecular Weight: 68,308 Da
• NCBI Official Full Name: mucin-20 isoform a
• NCBI Official Synonym Full Names: mucin 20, cell surface associated
• NCBI Official Symbol: MUC20
• NCBI Official Synonym Symbols: MUC-20
• NCBI Protein Information: mucin-20
• UniProt Protein Name: Mucin-20
• Protein Family: Mucin
• UniProt Gene Name: MUC20
• UniProt Synonym Gene Names: KIAA1359; MUC-20
• UniProt Entry Name: MUC20_HUMAN

NCBI Description

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The C-terminus of this family member associates with the multifunctional docking site of the MET proto-oncogene and suppresses activation of some downstream MET signaling cascades. The protein features a mucin tandem repeat domain that varies between two and six copies in most individuals. Multiple variants encoding different isoforms have been found for this gene. A related pseudogene, which is also located on chromosome 3, has been identified. [provided by RefSeq, Apr 2014]

Uniprot Description

MUC20: May regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF)-induced transient MAPK activation. Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: microvillus membrane; Golgi lumen; apical plasma membrane; basal plasma membrane; extracellular region

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; activation of MAPK activity; hepatocyte growth factor receptor signaling pathway; post-translational protein modification; protein homooligomerization