Human Syndecan 3 (SDC3) ELISA Kit | SDC3 elisa kit

Human Syndecan 3 (SDC3) ELISA Kit

Cat. Num. AAA2023601

• Gene Names: SDC3; SDCN; SYND3
• Reactivity: Human
• Synonyms: Syndecan 3 (SDC3); Human Syndecan 3 (SDC3) ELISA Kit; SDCN; SYND3; N-Syndecan; Syndecan Proteoglycan 3; SDC3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Syndecan 3 (SDC3).; No significant cross-reactivity or interference between Syndecan 3 (SDC3) and analogues was observed.
• Sequence Length: 442

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.226ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Syndecan 3 (SDC3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Syndecan 3 (SDC3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SDC3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Syndecan 3 (SDC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Syndecan 3 (SDC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Syndecan 3 (SDC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Syndecan 3 (SDC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SDC3 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 187960059
• NCBI GeneID: 9672
• NCBI Accession #: NP_055469.3
• NCBI GenBank Nucleotide #: NM_014654.3
• UniProt Accession #: O75056; Q5T1Z6; Q5T1Z7; Q96CT3; Q96PR8
• Molecular Weight: 45,497 Da
• NCBI Official Full Name: syndecan-3
• NCBI Official Synonym Full Names: syndecan 3
• NCBI Official Symbol: SDC3
• NCBI Official Synonym Symbols: SDCN; SYND3
• NCBI Protein Information: syndecan-3
• UniProt Protein Name: Syndecan-3
• Protein Family: Syndecan
• UniProt Gene Name: SDC3
• UniProt Synonym Gene Names: KIAA0468; SYND3
• UniProt Entry Name: SDC3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the syndecan proteoglycan family. It may play a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Allelic variants of this gene have been associated with obesity. [provided by RefSeq, Oct 2009]

Uniprot Description

syndecan-3: Cell surface proteoglycan that may bear heparan sulfate. May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Belongs to the syndecan proteoglycan family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.; Cell adhesion

Chromosomal Location of Human Ortholog: 1p35.2

Cellular Component: lysosomal lumen; membrane; Golgi lumen; integral to membrane; plasma membrane

Biological Process: chondroitin sulfate metabolic process; phototransduction, visible light; glycosaminoglycan biosynthetic process; extracellular matrix organization and biogenesis; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; retinoid metabolic process

Disease: Obesity