Human RAD23 Homolog B (RAD23B) ELISA Kit | RAD23B elisa kit

Human RAD23 Homolog B (RAD23B) ELISA Kit

Cat. Num. AAA2022513

• Reactivity: Human
• Synonyms: RAD23 Homolog B (RAD23B); Human RAD23 Homolog B (RAD23B) ELISA Kit; HHR23B; P58; HR23B; XP-C Repair Complementing Protein; XP-C Repair Complementing Complex 58 kDa; UV excision repair protein RAD23 homolog B; RAD23B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RAD23 Homolog B (RAD23B).; No significant cross-reactivity or interference between RAD23 Homolog B (RAD23B) and analogues was observed.
• Sequence Length: 416

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.052ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAD23 Homolog B (RAD23B) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAD23 Homolog B (RAD23B) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RAD23B elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAD23 Homolog B (RAD23B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAD23 Homolog B (RAD23B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAD23 Homolog B (RAD23B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RAD23 Homolog B (RAD23B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for RAD23B elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 71896193
• NCBI GeneID: 594966
• NCBI Accession #: NP_001025578.1
• NCBI GenBank Nucleotide #: NM_001030407.1
• NCBI Official Full Name: UV excision repair protein RAD23 homolog B
• NCBI Official Synonym Full Names: RAD23 homolog B, nucleotide excision repair protein
• NCBI Official Symbol: rad23b
• NCBI Protein Information: UV excision repair protein RAD23 homolog B
• Protein Family: Ubiquitin receptor