Human Interleukin 23 Receptor (IL23R) ELISA Kit | IL23R elisa kit

Human Interleukin 23 Receptor (IL23R) ELISA Kit

Cat. Num. AAA2022222

• Reactivity: Human
• Synonyms: Interleukin 23 Receptor (IL23R); Human Interleukin 23 Receptor (IL23R) ELISA Kit; IL23R elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 23 Receptor (IL23R).; No significant cross-reactivity or interference between Interleukin 23 Receptor (IL23R) and analogues was observed.
• Sequence Length: 335

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 23 Receptor (IL23R) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 23 Receptor (IL23R) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL23R elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 23 Receptor (IL23R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 23 Receptor (IL23R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 23 Receptor (IL23R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 23 Receptor (IL23R) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IL23R elisa kit

Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 211639210
• NCBI GeneID: 149233
• UniProt Accession #: B6VNT7; Q5VWK5
• Molecular Weight: 38,062 Da
• NCBI Official Full Name: interleukin 23 receptor, partial
• NCBI Official Synonym Full Names: interleukin 23 receptor
• NCBI Official Symbol: IL23R
• NCBI Protein Information: interleukin-23 receptor
• UniProt Protein Name: Interleukin 23 receptor
• Protein Family: Interleukin-23 receptor
• UniProt Gene Name: IL23R
• UniProt Entry Name: B6VNT7_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner. [provided by RefSeq, Jul 2008]

Uniprot Description

IL23R: Associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis. Genetic variations in IL23R are associated with inflammatory bowel disease type 17 (IBD17). IBD17 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Genetic variations in IL23R are a cause of susceptibility to psoriasis type 7 (PSORS7). Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. Belongs to the type I cytokine receptor family. Type 2 subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: receptor complex

Molecular Function: interleukin-23 receptor activity; interleukin-23 binding; interleukin-12 receptor binding

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of memory T cell differentiation; positive regulation of interleukin-17 production; positive regulation of natural killer cell proliferation; positive regulation of NK T cell activation; negative regulation of interleukin-10 production; positive regulation of T-helper 1 type immune response; positive regulation of interleukin-12 production; positive regulation of T cell mediated cytotoxicity; positive regulation of osteoclast differentiation; positive regulation of tyrosine phosphorylation of Stat4 protein; response to lipopolysaccharide; positive regulation of activated T cell proliferation; defense response to Gram-negative bacterium; regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of interferon-gamma production; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of T cell proliferation; positive regulation of defense response to virus by host; inflammatory response

Disease: Inflammatory Bowel Disease 17; Psoriasis 7, Susceptibility To