Human AE Binding Protein 1 (AEBP1) ELISA Kit | AEBP1 elisa kit

Human AE Binding Protein 1 (AEBP1) ELISA Kit

Cat. Num. AAA2022177

• Gene Names: AEBP1; ACLP
• Reactivity: Human
• Synonyms: AE Binding Protein 1 (AEBP1); Human AE Binding Protein 1 (AEBP1) ELISA Kit; ACLP; Aortic Carboxypeptidase-Like Protein; Adipocyte Enhancer Binding Protein 1; AEBP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of AE Binding Protein 1 (AEBP1).; No significant cross-reactivity or interference between AE Binding Protein 1 (AEBP1) and analogues was observed.
• Sequence Length: 733

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.78-50ng/mL
• Sensitivity: < 0.32ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AE Binding Protein 1 (AEBP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AE Binding Protein 1 (AEBP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AEBP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AE Binding Protein 1 (AEBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to AE Binding Protein 1 (AEBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AE Binding Protein 1 (AEBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AE Binding Protein 1 (AEBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for AEBP1 elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 119581523
• NCBI GeneID: 165
• UniProt Accession #: Q8IUX7; Q14113; Q59ER7; Q6ZSC7; Q7KZ79
• Molecular Weight: 82,183 Da
• NCBI Official Full Name: AE binding protein 1
• NCBI Official Synonym Full Names: AE binding protein 1
• NCBI Official Symbol: AEBP1
• NCBI Official Synonym Symbols: ACLP
• NCBI Protein Information: adipocyte enhancer-binding protein 1
• UniProt Protein Name: Adipocyte enhancer-binding protein 1
• Protein Family: Adipocyte enhancer-binding protein
• UniProt Gene Name: AEBP1
• UniProt Synonym Gene Names: ACLP; AE-binding protein 1
• UniProt Entry Name: AEBP1_HUMAN

NCBI Description

This gene encodes a member of carboxypeptidase A protein family. The encoded protein may function as a transcriptional repressor and play a role in adipogenesis and smooth muscle cell differentiation. Studies in mice suggest that this gene functions in wound healing and abdominal wall development. Overexpression of this gene is associated with glioblastoma. [provided by RefSeq, May 2013]

Uniprot Description

AEBP1: May positively regulate MAP-kinase activity in adipocytes, leading to enhanced adipocyte proliferation and reduced adipocyte differentiation. May also positively regulate NF-kappa-B activity in macrophages by promoting the phosphorylation and subsequent degradation of I- kappa-B-alpha (NFKBIA), leading to enhanced macrophage inflammatory responsiveness. Can act as a transcriptional repressor. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: extracellular matrix; extracellular space; cytoplasm; nucleus

Molecular Function: calmodulin binding; carboxypeptidase activity; zinc ion binding; transcription factor activity; metallocarboxypeptidase activity; transcription corepressor activity

Biological Process: muscle development; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; proteolysis; skeletal development