Human Troponin I Type 1, Slow Skeletal (TNNI1) ELISA Kit | TNNI1 elisa kit

Human Troponin I Type 1, Slow Skeletal (TNNI1) ELISA Kit

Cat. Num. AAA2021767

• Gene Names: TNNI1; TNN1; SSTNI
• Reactivity: Human
• Synonyms: Troponin I Type 1; Slow Skeletal (TNNI1); Human Troponin I Type 1; Slow Skeletal (TNNI1) ELISA Kit; TNN1; Troponin I; slow-twitch isoform; TNNI1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Troponin I Type 1, Slow Skeletal (TNNI1).; No significant cross-reactivity or interference between Troponin I Type 1, Slow Skeletal (TNNI1) and analogues was observed.
• Sequence Length: 187

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 46.88-3000pg/mL
• Sensitivity: < 17.59pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Troponin I Type 1, Slow Skeletal (TNNI1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Troponin I Type 1, Slow Skeletal (TNNI1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNNI1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin I Type 1, Slow Skeletal (TNNI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Troponin I Type 1, Slow Skeletal (TNNI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin I Type 1, Slow Skeletal (TNNI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Troponin I Type 1, Slow Skeletal (TNNI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TNNI1 elisa kit

Cardiovascular biology

NCBI and Uniprot Product Information

• NCBI GI #: 15214933
• NCBI GeneID: 7135
• UniProt Accession #: P19237; Q659A5; Q6FGS7; Q6FGW1; Q6ICU2; Q86T57; Q96DT9; A6NEH3; A8MSJ0
• Molecular Weight: 21,692 Da
• NCBI Official Full Name: Troponin I type 1 (skeletal, slow)
• NCBI Official Synonym Full Names: troponin I type 1 (skeletal, slow)
• NCBI Official Symbol: TNNI1
• NCBI Official Synonym Symbols: TNN1; SSTNI
• NCBI Protein Information: troponin I, slow skeletal muscle
• UniProt Protein Name: Troponin I, slow skeletal muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNI1
• UniProt Entry Name: TNNI1_HUMAN

NCBI Description

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq, Jul 2008]

Uniprot Description

TNNI1: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Belongs to the troponin I family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 1q31.3

Cellular Component: troponin complex; cytosol

Molecular Function: metal ion binding; actin binding

Biological Process: regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; muscle filament sliding